J4 ›› 2016, Vol. 15 ›› Issue (2): 19-22.

• 基础医学 • 上一篇    下一篇

断尾鼠精放置不同时间行ICSI对胚胎发育的影响

  

  1. (大理大学附属医院,云南大理671000)
  • 收稿日期:2015-05-11 出版日期:2016-02-15 发布日期:2016-02-15
  • 作者简介:于成和,检验师,主要从事生殖医学与实验室技术研究.
  • 基金资助:

    大理学院青年教师科研基金资助项目(KYQN201316)

Effect of Different Standing Time of Mouse Docking Sperm in ICSI on Mouse Embryonic Development

  1. (Affiliated Hospital of Dali University, Dali, Yunnan 671000, China)
  • Received:2015-05-11 Online:2016-02-15 Published:2016-02-15

摘要:

目的:鼠精断尾放置不同时间后注射入卵,评价其对胚胎发育潜能的影响。方法:实验分为10组:以立即注射为对照
组,以分别放置0.5、1、2、3、4、5、6、12、24 h后注射为9个实验组,比较实验组与对照组有关原核形成率、2-细胞形成率、4-细胞
形成率、桑葚胚形成率、囊胚形成率的差异。结果:放置6 h开始,原核形成率(68.4% vs. 82.5 %)、2-细胞形成率(83.3% vs.
96.0%)显著地低于对照组(P < 0.05);放置4 h开始,4-细胞形成率(59.8% vs. 79.8 %)显著地低于对照组(P < 0.05);放置2 h开
始,桑葚胚形成率(47.9% vs. 67.7 %)显著地低于对照组(P < 0.05);放置0.5 h开始,囊胚形成率(38.9% vs. 53.5%)显著地低于
对照组(P < 0.05),1 h开始,囊胚形成率(17.0% vs. 53.5%)极显著地低于对照组(P < 0.01),放置3 h,无囊胚形成。结论:鼠精
断尾放置一段时间后注射入卵,降低鼠胚发育潜能。

关键词: 断尾鼠精, 不同时间, 胚胎发育潜能

Abstract:

Objective: To evaluate the effect of different standing time of mouse docking sperm in intracytoplasmic sperm injection
(ICSI)on embryo development potential. Methods: Ten groups were divided by docking sperm standing time(0, 0.5, 1, 2, 3, 4, 5, 6,
12, 24 h). The rates of pronucleus formation, 2-cell formation, 4-cell formation, morulation and blastocysts formation were compared
between each experimental group and the control group. Results: The pronucleus formation rate(68.4% vs. 82.5%), and the 2-cells
formation rate(83.3% vs. 96.0%)were significantly lower in the standing 6 h group than those in the control group(P<0.05). The 4-
cells formation rate(59.8% vs. 79.8%)was significantly lower in the standing 4 h group than that in the control group(P<0.05).
Morulation rate(47.9% vs. 67.7%)was significantly lower in the groups with standing 2 h group than that in the control group(P<
0.05). The average blastocyst formation rate(38.9% vs. 53.5%)was significantly lower in all standing groups than that in the control
group(P<0.05). The Blastocyst formation rate dropped significantly as the standing time extension. No blastocyst was observed when
standing time longer than 3 h. Conclusion: Delay of mouse docking sperm ICSI reduced the development potential of mouse embryo.

Key words: docking sperm, different time, embryo development potential

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