Objective: To investigate the chemical composition of the fermentation broth of the traditional medicinal fungus
Fomitiporia punctata. Methods: The ethyl acetate extract of the fermentation broth of F. punctata was isolated and purified using
silica gel column chromatography, gel chromatography, liquid chromatography, and preparative HPLC. The structures of the isolated
compounds were identified by nuclear magnetic resonance spectroscopy and mass spectra. Results: Six compounds were isolated from
the fermentation broth of F. punctata, namely punctatin A （1）, 3β -hydroxycinnamolide （2）, Cyclo（L-Pro-L-Tyr） （3）, Cyclo-4-
hydroxy-（L-Pro-L-Leu） （4）, 5-hydroxymethyl furfural （5） and p-hydroxybenzoic acid （6）. Conclusion: Compound 1 is a new
compound, and compounds 1-6 are isolated from F. punctata for the first time.

Objective: To investigate the promotional effects of shikonin on the healing of skin wounds in rats and its impacts on the
TGF-β1/p38 MAPK signaling pathway. Methods: Forty male SD rats were used to establish full-thickness skin wound model, which
was randomly divided into 0.9% sodium chloride solution group, positive control group, low-dose shikonin group and high-dose
shikonin group. The area of skin wounds was measured and calculated on the day of modeling and on the 2nd, 4th, 6th, 8th and 10th
days after modeling, and the materials were taken after 10 d of drug administration. Hematoxylin and eosin staining was used to observe
the histopathological characteristics of rat skin wounds in each group. Immunohistochemistry was employed to detect the expression of
TGF-β1, integrin β1, and CD34 in the tissues. Western blotting was adopted to quantitatively detect the protein expression of TGF-β1,
p38MAPK, phosphorylated p38 MAPK, and CD34 in the tissues. Results: Compared with the 0.9% sodium chloride solution group, the
wound area was significantly reduced in the positive control group, low-dose shikonin group, and high-dose shikonin group. After
shikonin intervention, the histopathological lesions of rat wounds were markedly improved, and the positive rates of hair follicle stem cell
markers integrin β1 and CD34 in the wound tissues significantly increased. The protein expression levels of TGF-β1 and p38 MAPK
were also significantly elevated, and the differences was statistically significant （P<0.05）. Conclusion: Shikonin effectively promotes
skin wound healing in rats and improves the histopathological lesions of rat wounds, abd its mechanism may be related to the stimulation
of hair follicle stem cell marker expression and the activation of the TGF-β1/p38 MAPK signaling pathway.

Objective: To investigate the indigenous medicinal insect "Koudai Chong" used by the Jingpo ethnic group in western
Yunnan, aiming at identifying its zoological origin, usage methods, and medicinal efficacy. The research provides reference for the
exploration and documentation of traditional medicinal animal resources. Methods: Interviews with local people, field investigation,
specimen collections, morphological classification studies, and molecular biological techniques were employed to study the origin,
ecological environment, morphological characteristics, medicinal processing and usage methods, efficacy, and molecular markers of
"Koudai Chong". Results: The origin of "Koudai Chong" was identified as Atypus sacculatus, a species of the genus Atypus belonging to
the family Atypidae in the order Araneae. Its COI sequence length was found to be 637 bp. Traditionally, it is mainly used for treating
pediatric malnutrition （ganji）. Conclusion: This research provides significant evidence for the identification of the origin and the
medicinal development of "Koudai Chong", a traditional medicinal material used by the Jingpo ethnic group in western Yunnan.

Objective: To explore the potential mechanism of persicae semen-carthami flos medicine pair in the treatment of cervical
spondylosis. Methods: Active components of persicae semen-carthami flos were screened by databases such as TCMSP and PubChem,
and the targets of these components were identified using the Swiss Target Prediction database. Targets related to cervical spondylosis
were obtained from OMIM, DrugBank, and DisGeNET databases. Common targets of "the herb pair and the disease" were selected
using Venny software to construct a "drug-active ingredient-target-disease" network and a protein-protein interaction network,
thereby identifying core components and core targets. The Metascape platform was used for GO function and KEGG pathway
enrichment analysis of the core targets, and molecular docking validation was performed for some core components and core targets. Results: A total of 28 active components, 499 drug targets, and 5 093 disease targets were screened, yielding 312 common targets of
"the herb pair and the disease". GO function enrichment analysis revealed that persicae semen-carthami flos drug pair primarily
regulates responses to external stimuli and kinase activity to exert therapeutic effects. KEGG pathway enrichment analysis indicated
that the herb pair improves symptoms of cervical spondylosis by regulating pathways such as the PI3K-Akt signaling pathway, lipids,
and atherosclerosis. Molecular docking results showed good binding energies between lignin, luteolin, quercetin, and TP53, SRC,
PIK3CA. Conclusion: The persicae semen-carthami flos medicine pair effectively improves symptoms of cervical spondylosis through
a multi-component, multi-target, and multi-pathway mechanism. Signaling pathways such as PI3K-Akt may be the main route of
action, providing a theoretical basis for subsequent experimental research.

Objective: Weighted TOPSIS method was adopted to conduct a special review on the use of antitumor drugs in a hospital
and to provide reference for their rational application. Methods: A random sampling method was used to randomly select medical
records of discharged patients who used antitumor drugs from the clinical pharmacy management system of a hospital from July to
December 2022 （before intervention） and January to June 2023 （after intervention）. 270 medical records were selected for each time
period. The weighted TOPSIS method was used to evaluate the rationality of the use of antitumor drugs, and the rational use of
antitumor drugs before and after pharmaceutical intervention was compared. Results: Compared with that before intervention, the
rational use of antitumor drugs was significantly improved after the intervention （P<0.05）. The scores of seven evaluation indicators,
including tumor stage, ECOG or KPS score before chemotherapy, skip-level prescription and solvent, were significantly improved （P<
0.05）. Problems such as the use of proton pump inhibitors without indication, inappropriate duration of prophylaxis, and simultaneous
use of two 5-hydroxytryptamine 3 receptor antagonist were significantly reduced （P<0.05）. There were no medical records of using
neurokinin-1 （NK-1） receptor antagonist before the intervention, while 23 cases （accounting for 8.52%） used NK-1 receptor
antagonist after the intervention. Conclusion: Pharmaceutical intervention based on weighted TOPSIS method can promote the rational
use of antitumor drugs.

In view of the current situation that pharmacy courses rarely focus on the path of ideological and political education in
curriculum group to carry out in-depth and systematic research, the course team focuses on the core curriculum group of drug quality
research direction in the College of Pharmacy of Dali University, takes the resource base of ideological and political elements in mixed
teaching as the exploration direction of teaching reform, and builds a multi-dimensional practice path of ideological and political
education in curriculum group with full coverage. Through adding a teaching resource library of ideological and political elements,
optimizing teaching design and content supply and other reform measures, as well as establishing a multi-dimensional whole-process
assessment and evaluation system, the path of ideological and political construction of curriculum group is explored. It breaks the
previous fragmented state of ideological and political education between different courses, and promotes the same frequency resonance
and mutual penetration of curriculum group teaching and ideological and political education. It aims to cultivate students with a strong
concept of comprehensive quality control of drugs, practice the ideological and political goal of "educating people throughout the whole
process and in all aspects", and improve the effectiveness of education and teaching.

Objective: To investigate the effects of sacubitril valsartan sodium, dapagliflozin combined with finerenone on blood
glucose, blood pressure and renal function in type 2 diabetic nephropathy patients combined with hypertension. Methods: The clinical
data of 105 type 2 diabetic nephropathy patients combined with hypertension treated in the People′s Hospital of Xishuangbanna Dai
Autonomous Prefecture from February to December 2023 were retrospectively analyzed. Patients were divided into a control group and
an observation group according to different treatment regimens. The control group was treated with sacubitril valsartan sodium
combined with dapagliflozin, and the observation group was treated with finerenone on the basis of the control group. After 6 months of
continuous treatment, the blood glucose, blood pressure, renal function, plasma albumin and serum potassium levels of the two groups
were compared before and after treatment. Results: After 6 months of treatment, there were no obvious adverse reactions in two groups.
The blood pressure and blood glucose control in both groups improved significantly compared with that before treatment, and renal
function was significantly restored, and the blood pressure, renal function and plasma albumin control in the observation group were
better than those in the control group, and the differences were statistically significant （P<0.05）. Conclusion: Type 2 diabetic
nephropathy patients with hypertension were treated with finerenone on the basis of using sacubitril valsartan sodium and dapagliflozin,
which can effectively control the blood glucose and blood pressure of patients, delay the progress of nephrosis, and have positive
significance in protecting renal function.

Objective: To explore the clinical efficacy of Yiqi Yangyin Huoxue Decoction in treating patients with angina pectoris of
coronary heart disease. Methods: A total of 100 patients with angina pectoris of coronary heart disease admitted to the First Affiliated
Hospital of Guizhou University of Traditional Chinese Medicine from April 2023 to April 2024 were selected as the research subjects.
The patients were randomly divided into a control group （treated with conventional Western medicine） and an experimental group
（treated with a combination of conventional Western medicine and Yiqi Yangyin Huoxue Decoction） using a random number table
method. Each group had 50 patients and was treated continuously for 4 weeks. The clinical efficacy, occurence of adverse reactions,
attack of angina pectoris, cardiac function and vascular endothelial function, serum inflammatory factor levels, and changes in
hemorheological indicators were compared between the two groups. Results: The total effective rate of the experimental group was
significantly higher than that of the control group, and the experimental group was significantly better than the control group in
improving attack of angina pectoris, cardiac function, and endothelial function. The serum inflammatory factor contents of patients in
the experimental group were significantly lower than those of the control group, and the improvement of hemorheological indicators was
significantly better than that of the control group, with statistically significant difference （P<0.05）. Conclusion: The combination of
Yiqi Yangyin Huoxue Decoction and conventional Western medicine has significant therapeutic effects on angina pectoris of coronary
heart disease, it can reduce inflammation, improve heart function, endothelial function, and hemorheological indicators.

Objective: To explore the clinical efficacy of Shiwei Wendan Decoction combined with western medicine in the treatment
of hypertension accompanied by insomnia. Methods: A total of 60 patients diagnosed with phlegm dampness internal resistance type
hypertension accompanied by insomnia at the outpatient clinic of the First Affiliated Hospital of Guizhou University of Traditional
Chinese Medicine from January 2023 to January 2024 were enrolled in the study. Patients were divided into a contrd group and an
experiment group according to random number table method, with 30 cases in each group. The control group received nifedipine control
release tablets combined with estazolam tablets, while the experimental group received nifedipine control release tablets combined with
Shiwei Wendan Decoction. Changes in blood pressure, Pittsburgh sleep quality index （PSQI） scores, Traditional Chinese Medicine
（TCM） syndrome scores, efficacy, and adverse reactions before and after treatment were compared between the two groups. Results:
After treatment, both groups showed significant reductions in blood pressure, PSQI scores, and TCM syndrome scores compared to
before treatment. The experimental group showed greater improvement in all these indicators compared to the control group. The total
effective rate in the experimental group was significantly higher than that in the control group, with statistically significant differences
（P<0.05）. The incidence of adverse reactions in both groups was 3.33%, with no significant difference （P>0.05）. Conclusion: Shiwei
Wendan Decoction demonstrates a remarkable clinical efficacy with fewer adverse reactions, making it effective in improving insomnia
symptoms in patients with hypertension.

Objective: To investigate the effects of slow intravenous infusion of low-dose mannitol on cardiac troponin T （cTnT）, Creactive
protein （CRP）, tumor necrosis factor-α （TNF-α）, and interleukin-6 （IL-6） levels after cardiopulmonary bypass （CPB）
cardiac surgery, and to evaluate the efficacy of this therapeutic strategy in mitigating postoperative adverse reactions. Methods: A total
of 71 patients who underwent CPB cardiac surgery at the Third Affiliated Hospital of Xinxiang Medical University from June 2020 to
March 2022 were included in the study. Patients were randomly divided into a control group and an experimental group. The control
group received conventional treatment, while the experimental group received additional low-dose mannitol infusion therapy based on
conventional treatment. The levels of cTnT, CRP, TNF-α, and IL-6 were compared at different time points postoperatively between the
two groups. Results: The levels of cTnT, CRP, TNF-α, and IL-6 increased initially and then decreased in both groups postoperatively,
with peak levels observed at 36 hours, 2 days, 6 hours, and 12 hours postoperatively, respectively. There were statistically significant
differences in intergroup comparisons, time effects, and trends of change（ P<0.05）. The peak levels of these indicators were lower, and
the decrease rate was faster in the experimental group. Conclusion: Slow intravenous infusion of low-dose mannitol can effectively
reduce the levels of cTnT, CRP, TNF-α, and IL-6 after CPB cardiac surgery, alleviate postoperative adverse reactions, and holds
clinical promotional value.

Objective: To investigate the expression of acetyltransferase KAT2A and the acetylation of Survivin protein in esophageal
cancer tissues. Methods: A total of 60 esophageal cancer patients diagnosed at the First Affiliated Hospital of Dali University between
March 2020 and March 2022 were included in this study. Cancer tissues were collected as the observation group, and normal
esophageal epithelial tissues, 8 cm away from the cancer site without cancer invasion, were collected as the control group. The
expression levels of Survivin and KAT2A, as well as the acetylation level of Survivin protein, were measured and compared between the
groups. The correlation between Survivin protein acetylation and KAT2A expression was also analyzed. Results: The relative
expression levels of Survivin mRNA and KAT2A mRNA, the expression levels of Survivin protein and KAT2A protein, and the
acetylation rate of Survivin in the observation group were all significantly higher than those in the control group, and the differences
were statistically significant（ P<0.01）. There was a significant positive correlation between Survivin acetylation and KAT2A expression
in esophageal cancer （r=0.543）. Significant differences were found in the positive rates of Survivin acetylation and KAT2A expression
among patients with different stages, different degrees of differentiation, and with or without lymph node metastasis （P<0.05）.
Conclusion: KAT2A is involved in the acetylation modification of Survivin protein in patients with esophageal cancer, and the high
expression of KAT2A may be an early molecular event in the acetylation modification of Survivin protein.

Objective: To investigate the risk factors and resistance gene characteristics of carbapenem resistant Klebsiella
pneumoniae（ CRKP） infections in the Chongming Branch of Shanghai Tenth People′s Hospital. Methods: Clinical data were collected
from 103 patients with CRKP infections and 327 patients with non-CRKP infections between June 2017 and June 2023. Multivariate
Logistic regression analysis was used to identify the risk factors for CRKP infections. PCR was employed to detect the expression of
resistance and virulence genes in CRKP strains, and capsular serotyping was performed. Results: The result of multivariate Logistic
regression analysis showed that using ≥3 antimicrobial agents, mechanical ventilation, using urinary catheters, having ≥3 kinds of
underlying diseases, and having serum albumin levels <30 g/L were independent risk factors for patients to develop CRKP infection （P
<0.05）. There were 73 strains （accounting for 70.87%） carrying the KPC gene, 12 strains （accounting for 11.65%） carrying the NDM
gene, 7 strains （accounting for 6.80%） carrying the VIM gene, and 4 strains （accounting for 3.88%） carrying the OXA-48 gene.
Virulence gene detection revealed the following rates: rmpA （16.50%）, rmpA2 （18.45%）, iroN （17.48%）, iroB （12.62%）, peg-344
（8.74%）, and iutA （18.45%）. Conclusion: CRKP infections in hospitalized patients are influenced by multiple factors, with KPC,
NDM, and VIM being the predominant resistance genes. Some strains also carry high-virulence genes. Clinical efforts should be
enhanced to prevent infections in high-risk populations and to curb bacterial transmission.

Objective: To investigate the impact of serum hepatocyte growth factor （HGF）, urokinase plasminogen activator （uPA）,
interleukin-17 （IL-17）, and interleukin-24 （IL-24） on the prognosis of patients with oral squamous cell carcinoma （OSCC）.
Methods: A retrospective analysis was conducted on clinical data from 75 OSCC patients. Preoperative serum levels of HGF, uPA, IL-
17, and IL-24 were measured, and the differences in these biomarkers among patients with varying clinical characteristics were
compared. Kaplan-Meier survival analysis and COX regression models were used to assess the impact of these biomarkers on
prognosis. Receiver operator characteristic curves were employed to evaluate the diagnostic efficacy of each biomarker. Results:
Kaplan-Meier analysis and COX regression results indicated that the degree of differentiation, clinical stage, and the levels of the
aforementioned serum biomarkers were independent factors influencing OSCC prognosis. The area under the curve values for each
biomarker′s prognostic prediction were relatively high. Conclusion: Serum levels of HGF, uPA, IL-17, and IL-24 are closely related
to the prognosis of OSCC patients and can serve as important prognostic indicators. Integrating these biomarkers with clinical
characteristics helps in predicting prognosis and guiding personalized treatment.

Objective: To evaluate the diagnostic value of serum markers in the staging of chronic HBV infection throughout its
natural history. Methods: A retrospective analysis was conducted on the clinical data of 172 patients with chronic HBV infection, who
had not undergone antiviral treatment, collected from November 2020 to December 2023 at Kunming Third People′s Hospital . Based
on the natural history of chronic HBV infection, patients were classified into four groups: EPI, EPH, ENI, and ENH. Changes in serum
marker levels were compared among these groups, and the diagnostic value of each marker for the EPH and ENH stages was assessed
using ROC curve analysis. Results: Significant differences were observed among groups in age, RDW-SD, HBeAg, HBsAg, HBV
DNA, HBV RNA, ALT, AST, GGT, PT, PTA, APRI, FIB-4, and GPR levels （P<0.05）. Univariate analysis for HBeAg-positive and
HBeAg-negative patients revealed significant differences between the EPI and EPH groups in age, Hb, HBeAg, HBsAg, ALT, AST,
GGT, AFP, APRI, FIB-4, and GPR levels（ P<0.05）. Similarly, significant differences were found between the ENI and ENH groups in
HBV DNA, HBV RNA, ALT, AST, GGT, APRI, and GPR levels（ P<0.05）. ROC curve analysis indicated that the combined diagnostic
prediction using multiple serum markers had a higher diagnostic efficiency for the EPH and ENH stages compared to individual
markers. Conclusion: The combined diagnosis of multiple serum markers has high clinical value and is expected to provide
noninvasive diagnostic support for the staging of chronic HBV infection and the selection of antiviral therapy.

Objective: To understand the current situation of electronic health literacy of college students from a University in Dali
area, and to explore its influencing factors. Methods: A total of 1 114 college students from a University in Dali area were selected
from June to November 2023 by cluster random sampling method for electronic health literacy survey. Results: The total score of
electronic health literacy of the 1 114 college students was （29.28±6.07）, and the qualified rate was 22.40%. The results of Logistic
regression analysis showed that the average monthly living expenses, father′s occupation, mother′s occupation, whether the only child,
whether the students ate on time, the regularity of their life and work, sleep quality, and whether they participated in the activities of
health-related clubs were the electronic health literacy influencing factors（P＜0.05）. Conclusion: The level of electronic health
literacy among college students from a University in Dali area needs to be improved. Colleges and universities should strengthen the
publicity of health education and improve college students′ ability to judge and make decisions on online health information.

Objective: To investigate the status quo and influencing factors of readiness for hospital discharge of patients with lumbar
spinal stenosis undergoing surgery. Methods: A questionnaire survey was conducted on 220 patients with lumbar spinal stenosis who
were admitted to the spinal surgery department of a tertiary hospital in Dali city from October 2022 to June 2023 by convenient
sampling method. Results: The total score of readiness for hospital discharge of 220 patients with lumbar spinal stenosis undergoing
surgery was （90.77±8.25）. The results of multiple linear regression analysis showed that gender, education level, reason for discharge,
quality of discharge instructions, and social support were the influencing factors of readiness for hospital discharge of patients with
lumbar spinal stenosis undergoing surgery （P＜0.05）. Conclusion: The readiness for hospital discharge of patients with lumbar spinal
stenosis undergoing surgery is at a medium level. Healthcare professionals should strengthen discharge education for female patients,
patients with lower education level and patients whose doctors recommend discharge. Healthcare professionals should also formulate
targeted interventions for these patients to improve their readiness for discharge and social adaptation.

Objective: To construct smart elderly care community and service model innovation strategy, and evaluate the effect of its
application in the management of elderly chronic disease patients in a community in Fujian Province. Methods: A total of 1 356
elderly patients with chronic diseases in a community in Fujian Province from January to December 2023 were selected as the
observation group, and 1 474 elderly patients with chronic diseases in a nearby community were selected as the control group. The
management of the observation group adopted the smart elderly care community and service model innovation strategy, and the control
group received the conventional community management of chronic diseases. The effect of blood pressure, blood glucose and blood
lipids control effect and attainment rate changes were compared, as well as changes in lifestyle, disease awareness rate, management
satisfaction degree. Results: After one year′s intervention, the systolic blood pressure, diastolic blood pressure, fasting plasma glucose,
2 hour postprandial plasma glucose, triglyceride, total cholesterol, low-density lipoprotein cholesterol, smoking rate and drinking rate
in the observation group were significantly lower than those in the control group （P<0.05）. The high-density lipoprotein cholesterol,
the control rates of blood pressure, blood glucose and blood lipids, regular exercise rate, the awareness rates of hypertension, diabetes
and hyperlipidemia, and management satisfaction degree in the observation group were significantly higher than those in the control
group （P<0.05）. Conclusion: The smart elderly care community and innovative strategy of service model can effectively improve the
management level and attainment rate of patients′ blood pressure, blood glucose and blood lipids, and help patients maintain good
living habits, improve the awareness rate of chronic diseases and management satisfaction degree.

Objective: To investigate the application effect of E-Portfolio combined with BOPPPS teaching model in obstetrics and
gynecology nursing course. Methods: A total of 208 full-time nursing students in grade 2022 from a vocational college were selected
as the study objects and randomly divided into a control group and an observation group, with 104 students in each group. The control
group adopted the traditional teaching method, and the observation group implemented the E-Portfolio combined with BOPPPS
teaching model. At the end of the course, the students performed self-evaluation of their post competence and clinical thinking ability,
and were assessed on theoretical knowledge, case analysis and practical training operation, as well as satisfaction evaluation of the
teaching methods. Results: The post competence, clinical thinking ability, assessment results of theoretical knowledge, case analysis
and practical training operation, and satisfaction with teaching methods in the observation group were higher than those in the control
group, and the differences were statistically significant （P<0.05）. Conclusion: The application of E-Portfolio combined with the
BOPPPS teaching model in obstetrics and gynecology nursing course can significantly improve students′ post competence and clinical
thinking ability, and obtain a high degree of satisfaction from students, which provides a useful reference for the teaching model of
medical professional courses in the future.