粪样保存方法对黑白仰鼻猴肠道结节线虫监测的影响

›› 2017, Vol. 2 ›› Issue (12): 70-73.

粪样保存方法对黑白仰鼻猴肠道结节线虫监测的影响

（1.大理大学东喜玛拉雅研究院，云南大理671003；2.云南省中国三江并流区域生物多样性协同创新中心，云南大理671003；3.白马雪山国家自然保护区管理局，云南迪庆674609；4.西南林业大学，昆明650224）

收稿日期:2017-08-25 出版日期:2017-12-15 发布日期:2017-12-15
通讯作者: 肖文，研究员，博士.
作者简介:李延鹏，研究实习员，主要从事灵长类行为生态学研究.
基金资助:
国家自然科学基金资助项目（31260149；31560118）；云南省中青年学术技术带头人后备人才资助项目（2014HB024）

Effects of Fecal Sample Preservation Methods on the Monitoring of Intestinal Oesophagostomum in Rhinopithecus bieti

（1. Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China; 2. Collaborative Innovation Center for the Biodiversity in the Three Parallel Rivers of China，Dali, Yunnan 671003, China; 3. Administration of Baimaxueshan National Nature Reserve, Diqing, Yunnan 674609, China; 4. Southwest Forestry University, Kunming 650224, China）

Received:2017-08-25 Online:2017-12-15 Published:2017-12-15

摘要/Abstract

摘要： 目的：探索一种有效的黑白仰鼻猴肠道寄生虫虫卵定量监测的粪便样品保存方法。方法：对两种保存方法（福尔马林保存液保存和冷冻保存）下的黑白仰鼻猴粪便样品进行寄生虫虫卵检出率及检出强度进行对比检测，分析不同保存条件下的粪便保存效果。结果：-20 ℃冷冻条件下保存的粪便样品，结节线虫虫卵检出强度为（919.69±916.09）个/g，检出率是88.89%（56/63），检出率在30 d内没有下降，检出强度也维持在较高的水平上。福尔马林液保存的粪便样品在第4天时结节线虫的检出强度就下降至4.98%，检出强度及检出率都呈现显著的差异（t=6.799，P <0.001，df =62；χ2 =45.10，P <0.01）。结论：将黑白仰鼻猴粪便置于-20 ℃冷冻条件下保存，在保证结节线虫检出率及检出强度的情况下，可有效延长保存时间。

关键词: 黑白仰鼻猴, 粪便样品, 保存方法, 检出率, 检出强度

Abstract: Objective: To explore an effective fecal sample preservation method for quantitative monitoring of intestinal Oesophagostomum in Rhinopithecus bieti. Methods: The detection rate and detection intensity of Oesophagostomum in Rhinopithecus bieti fecal samples from two preservation methods（formalin preservation and cryopreservation）were compared and analyzed, and the sample preservation under different storage conditions was analyzed for different effects. Results: The samples stored at -20 ℃ showed that the detection intensity of Oesophagostomum eggs was（919.69 ± 916.09）/ g, the detection rate was 88.89%（56/63）, the detection rate did not decline within 30 days, and the detected intensity also maintained at a high level. The sample stored in formalin reduced its intensity to 4.98% on 4th day, and the detection intensity and detection rate showed significant differences（t=6.799, P <0.001, df=62; χ2=45.10, P<0.01）. Conclusion: In a frozen condition at -20 ℃ which ensures the detection rate and intensity of Oesophagostomum, the storage time of the fecal samples of Rhinopithecus bieti can be effectively prolonged.

Key words: Rhinopithecus bieti, fecal samples, preservation method, detection rate, detection intensity

中图分类号:

李延鹏，黄志旁，刘硕然，杨毅梅，钟泰，苏锡钧，崔亮伟，肖文. 粪样保存方法对黑白仰鼻猴肠道结节线虫监测的影响[J]. , 2017, 2(12): 70-73.

Li Yanpeng, Huang Zhipang, Liu Shuoran, Yang Yimei, Zhong Tai, Su Xijun,. Effects of Fecal Sample Preservation Methods on the Monitoring of Intestinal Oesophagostomum in Rhinopithecus bieti[J]. , 2017, 2(12): 70-73.

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