ARα调控SOD2表达的机制研究

doi:10. 3969 / j. issn. 2096-2266. 2018. 02. 007

大理大学学报

ARα调控SOD2表达的机制研究

（1.大理大学基础医学院，云南大理671000；2. 大理大学第一附属医院，云南大理671000）

收稿日期:2017-06-08 出版日期:2018-02-15 发布日期:2018-02-15
作者简介:杨锡彤，硕士研究生，主要从事病理学与病理生理学研究.
基金资助:
国家自然科学基金资助项目（81360206）；云南省中青年学术技术带头人后备人才资助项目（2014HB025）

Exploration on the Mechanism of PPARα on Regulating the SOD2 Expression

（1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China; 2. The First Affiliated Hospital of Dali University, Dali,
Yunnan 671000, China）

Received:2017-06-08 Online:2018-02-15 Published:2018-02-15

摘要/Abstract

摘要： 目的：探究PPARα调控SOD2表达的机制。方法：对脑微血管内皮细胞（BMEC）和C57BL/6小鼠给予非诺贝特处理，然
后用实时荧光定量PCR检测BMEC和经非诺贝特处理的C57BL/6小鼠分离的脑微血管中的PPARα（过氧化物酶体增殖物激活
受体α亚型）和SOD2（锰超氧化物歧化酶）的表达；构建pGL4 mSOD2（含SOD2基因启动子序列）或pGL4 mu mSOD2即含SOD2
基因启动子中PPRE（过氧化物酶体增殖物反应元件）结合区域突变荧光素酶报告质粒，分别转染BMEC后检测细胞中的荧光
素酶活性。结果：经非诺贝特处理C57BL/6小鼠的脑微血管和BMEC中，其PPARα和SOD2的表达均升高；经非诺贝特处理后
荧光素酶活性增强，但是SOD2基因启动子PPRE结合序列突变后，给予非诺贝特处理其荧光素酶的活性也未见改变。结论：
非诺贝特通过激活PPARα，激活的PPARα与SOD2基因启动子上游的PPRE结合以调节SOD2的表达。

关键词: 非诺贝特, 过氧化物酶体增殖物激活受体, 超氧化物歧化酶

Abstract: Objective: To investigate the mechanism of PPARα on regulating the SOD2 expression. Methods: The BMEC and the
mice C57BL/6 were treated with fenofibrate. PPARα and SOD2 expression in BMEC and the brain microvessel of the mice C57BL/6
which treated with fenofibrate were tested by the real-time PCR. Luciferase reporter plasmid including the promoter of SOD2 was
named as pGL4 mSOD2 and the promoter of SOD2 which PPRE domain was mutated was named as pGL4 mu mSOD2. The activity of
Luciferase in BMEC which was co-transfected with Luciferase reporter system including pGL4 mSOD2, or pGL4 mu mSOD2 with
pGL4 was tested. Results: The expression of PPARα and SOD2 were enhanced in the BMEC or mice C57BL/6 treated with fenofibrate.
The activity of Luciferase in BMEC was promoted in the Luciferase reporter system including pGL4 mSOD2 when the fenofibrate was
used; however, there was no change of the activity of Luciferase in the Luciferase reporter system including pGL4 mu mSOD2 even
though the BMEC was treated with fenofibrate. Conclusion: Fenofibrate regulates the SOD2 expression by activating PPARα,
activated PPARα combine with pGL4 mSOD2 PPRE.

Key words: fenofibrate, peroxisome proliferator-activated receptor, superoxide dismutase

杨锡彤，秦燕，杜小珊，徐弘扬，王光明. ARα调控SOD2表达的机制研究[J]. 大理大学学报, DOI: 10. 3969 / j. issn. 2096-2266. 2018. 02. 007.

Yang Xitong, Qin Yan, Du Xiaoshan, Xu Hongyang, Wang Guangming. Exploration on the Mechanism of PPARα on Regulating the SOD2 Expression[J]. Journal of Dali University, DOI: 10. 3969 / j. issn. 2096-2266. 2018. 02. 007.

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ARα调控SOD2表达的机制研究

Exploration on the Mechanism of PPARα on Regulating the SOD2 Expression

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