C3H10T1/2细胞黏着斑与微丝及细胞面积的相关性研究

大理大学学报 ›› 2019, Vol. 4 ›› Issue (12): 75-78.

C3H10T1/2细胞黏着斑与微丝及细胞面积的相关性研究

大理大学工程学院，云南大理671003

收稿日期:2018-08-16 出版日期:2019-12-15 发布日期:2019-12-15
作者简介:李司婧，助教，主要从事组织工程研究.
基金资助:
大理大学青年教师科研基金资助项目（KYQN201434）

Study on the Correlation among Focal Adhesion and Microfilaments and Cell Areas of C3H10T1/2

（College of Engineering, Dali University, Dali, Yunnan 671003, China）

Received:2018-08-16 Online:2019-12-15 Published:2019-12-15

摘要/Abstract

摘要： 黏着斑是将细胞外基质与细胞骨架联系起来的多蛋白聚集体，可形成细胞迁移的伪足。微丝对细胞贴附、铺展、运动、
内吞、细胞分裂等许多细胞功能具有重要作用。对间充质干细胞（C3H10T1/2）骨架系统进行研究，通过采用ZEN、ImageJ以及
SPSS等软件分析黏着斑、微丝以及细胞面积之间的关系。实验结果显示，微丝总长度与黏着斑数量、黏着斑面积与黏着斑数
量、细胞面积与黏着斑数量均存在显著的相关关系。

关键词: 间充质干细胞, 细胞骨架, 微丝, 黏着斑

Abstract: Focal Adhesion is a multi protein aggregate linked by extracellular matrix and cytoskeleton, forming the pseudopodium for
cell migrations. Microfilaments play an important role in cell adhesion, spreading, movement, endocytosis, cell division and many other
cell functions. This paper studies the cytoskeleton system of C3H10T1/2 cells through analyzing the correlation between Focal
Adhesion and microfilaments and cell areas based on ZEN, ImageJ and SPSS. The results show the significant correlation between the
number of Focal Adhesion and total length of microfilaments, of the area of the plaque and the number of Focal Adhesion, and of the
cell area and the number of focal Adhesion.

Key words: mesenchymal stem cell, cytoskeleton, microfilament, focal adhesion

李司婧，董红丽. C3H10T1/2细胞黏着斑与微丝及细胞面积的相关性研究[J]. 大理大学学报, 2019, 4(12): 75-78.

Li Sijing, Dong Hongli. Study on the Correlation among Focal Adhesion and Microfilaments and Cell Areas of C3H10T1/2[J]. Journal of Dali University, 2019, 4(12): 75-78.

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