大理大学学报 ›› 2021, Vol. 6 ›› Issue (4): 14-17.

• 药学 • 上一篇    下一篇

滇西18种豆科植物体外降脂活性研究

 郎利娟,姜 北,崔淑君,朱起杰,董 相,肖朝江   

  1. (1.大理大学药物研究所,云南大理 671000;2.大理大学药学院,云南大理 671000)
  • 收稿日期:2020-12-02 修回日期:2020-12-13 出版日期:2021-04-15 发布日期:2021-05-19
  • 通讯作者: 肖朝江,副研究员,博士,E-mail:xiaochaojiang@yeah.net。
  • 作者简介:郎利娟,硕士研究生,主要从事天然产物研究。
  • 基金资助:
    云南省地方本科高校(部分)基础研究联合专项资金项目(2019FH001-050);大理大学滇西特色药用植物与白
    族药开发利用创新团队项目(ZKLX2019106);大理大学博士科研启动费项目(KYBS201734)

In vitro Lipid-Lowering Activity of 18 Leguminosae Plants in West Yunnan

 Lang Lijuan, Jiang Bei, Cui Shujun, Zhu Qijie, Dong Xiang, Xiao Chaojiang   

  1. (1.Institute of Materia Medica, Dali University, Dali, Yunnan 671000, China; 2.College of Pharmacy, Dali University,
    Dali, Yunnan 671000, China)
  • Received:2020-12-02 Revised:2020-12-13 Online:2021-04-15 Published:2021-05-19

摘要: [摘要]目的:研究滇西 18 种豆科植物的体外降脂活性,为发现植物来源的新型降脂天然产物奠定基础。方法:利用
75% 乙醇对 18 种豆科植物进行热回流提取;采用高脂 HepG2 细胞模型对各植物提取物进行体外降脂活性筛选,通过
降脂率对其活性进行评价。结果:13 种植物提取物具有不同程度的降脂活性,其中云南甘草地上部分、截叶铁扫帚全
株、黑毛多枝黄芪地下部分、鞍叶羊蹄甲地上部分提取物的降脂活性较好,终浓度为0.5 mg/mL时的降脂率分别为(21.6±
3.9)%、(19.6±10.0)%、(17.8±1.9)%、(15.3±4.6)%。结论:云南甘草地上部分、截叶铁扫帚全株、黑毛多枝黄芪地下部分、鞍叶
羊蹄甲地上部分具有较好的降脂活性,值得后续深入研究。

关键词: [关键词]豆科, 体外降脂活性, 高脂HepG2细胞模型, 云南甘草

Abstract: 〔Abstract〕Objective: To explore the in vitro lipid-lowering activity of 18 Leguminosae plants in west Yunnan, and to provide a
scientific basis for the discovery of novel hypolipidemic drugs from plants. Methods: 18 Leguminosae plants were extracted with
75% ethanol by heat reflux. High-fat HepG2 cell model was used to screen the lipid-lowering activity of the crude extracts in vitro,
and the bioactive results were evaluated by lipid-lowering rates. Results: 13 plant extracts showed lipid-lowering activity in
different degrees. Among them, the aerial parts of Glycyrrhiza yunnanensis, the whole herb of Lespedeza cuneata, the underground
parts of Astragalus polycladus var. nigrescens and the aerial parts of Bauhinia brachycarpa, showed significant activities to inhibit
lipid accumulation, with inhibition ratios of (21.6±3.9)% , (19.6±10.0)% , (17.8±1.9)% and (15.3±4.6)% , respectively, at
concentration of 0.5 mg/mL. Conclusion: The aerial parts of Glycyrrhiza yunnanensis, the whole herb of Lespedeza cuneata, the
underground parts of Astragalus polycladus var. nigrescens and the aerial parts of Bauhinia brachycarpa, have good lipid-lowering
activity, which is worth a further study.

Key words: 〔Key words〕Leguminosae, in vitro lipid-lowering activity, high-fat HepG2 cell model, Glycyrrhiza yunnanensis

中图分类号: