大理大学学报 ›› 2023, Vol. 8 ›› Issue (8): 54-58.

• 基础医学 • 上一篇    下一篇

高盐干预对人主动脉血管平滑肌细胞增殖和迁移的影响

邓文斌,黄仕琼,王 雪,李利华*   

  1. (大理大学第一附属医院老年病科,云南大理 671000

  • 收稿日期:2022-11-11 修回日期:2023-01-20 出版日期:2023-08-15 发布日期:2023-07-11
  • 通讯作者: 李利华,教授,博士,E-mail:lilihuayncn@163.com。
  • 作者简介:邓文斌,硕士研究生,主要从事动脉硬化研究。
  • 基金资助:
    国家自然科学基金项目(8186008482260076

Effects of High Salt Intervention on Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cell

Deng WenbinHuang Shiqiong Wang XueLi Lihua*   

  1. Department of Geriatrics The First Affiliated Hospital of Dali University Dali Yunnan 671000 China

  • Received:2022-11-11 Revised:2023-01-20 Online:2023-08-15 Published:2023-07-11

摘要: 目的:研究不同浓度Na+干预对人主动脉血管平滑肌细胞(HAVSMC)增殖和迁移的影响。方法:原代培养HAVSMC,传代至第5代后用不同浓度的Na+132137142147152157162167 mmol/L)干预,培养24487296 h后进行细胞增殖和迁移实验。CCK-8法检测HAVSMC的增殖活性;实时荧光定量聚合酶链反应检测增殖细胞核抗原(PCNA)表达,分析HAVSMC的增殖能力;划痕实验评估HAVSMC的迁移能力。结果:与132 mmol/L Na+对照组相比,培养72 h后,137167 mmol/L Na+干预均可促进HAVSMC的增殖能力(P<0.05),Na+浓度为152 mmol/L时具有显著的促增殖作用(P<0.05),且可促进 HAVSMC的迁移。结论:高盐干预可促进HAVSMC增殖和迁移。

关键词: font-family:宋体, ">盐;人主动脉血管平滑肌细胞;增殖;迁移

Abstract: Objective To investigate the effects of different concentrations of Na+ intervention on the proliferation and migration of human aortic vascular smooth muscle cellHAVSMC. Methods HAVSMC were cultured as primary cultures and were passaged to the fifth generation. Cells were intervened with different concentrations of Na+132 137 142 147 152 157 162 and 167 mmol/L), and cell proliferation and migration experiments were conducted at 24 48 72 and 96 hours after the intervention. Proliferation activity of HAVSMC was detected by the CCK-8 method and proliferating cell nuclear antigenPCNA expression was analyzed by real-time fluorescence quantitative polymerase chain reaction in order to evaluate proliferation ability of HAVSMC. Additionally scratch assay was employed to evaluate the migration ability of HAVSMC. Results Compared with the 132 mmol/L Na+ control group after 72 hours of cultivation 137-167 mmol/L Na+ intervention promoted the proliferation ability of HAVSMC. Among all intervention groupsP<0.05), Na+ concentration of 152 mmol/L had a significant pro-proliferation effectP<0.05 and promoted the migration of HAVSMC. Conclusion High salt intervention can promote the proliferation and migration of HAVSMC.

Key words: font-family:Times New Roman, ">saltfont-family:Times New Roman, ">;font-family:Times New Roman, "> human aortic vascular smooth muscle cellfont-family:Times New Roman, ">;font-family:Times New Roman, "> proliferationfont-family:Times New Roman, ">;font-family:Times New Roman, "> migration

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