J4 ›› 2011, Vol. 10 ›› Issue (6): 51-53.
目的：检测核转录因子(NF—κB)在子宫内膜异位症(EM)患者异位内膜及在位内膜组织中的表达，以及其血清中肿瘤
坏死因子-α (TNF-α)的变化，探讨NF-κB与TNF-α在EM发病机制中的作用。方法：采用酶联免疫吸附试验(ELISA)测定TNF-α 的表达，免疫组化sP法检测NF-κB的表达情况。结果：子宫内膜异位症(EM)组血清TNF-α含量显著高于对照组(P<0．01)，且表达量随病情加重有上升趋势(P<0．05)。EM患者中异位内膜组NF-κB表达强度显著高于在位组及对照组(P<0．01)。结论：NF-κB与TNF-α在EM患者异位内膜中的异常高表达与EM的发病有密切关系。
0bjective：The expression of NF-κB in the tissue of endometria and tumor necrosis factor- α in serum in endometriosis patients were measured to investigate the relationship between NF-κB．TNF-α and endometriosis．Methods：Enzyme linked im munosorbent assay method was applied to detect the the expression of TNF-α．The expression of NF-κB was detected by immunohis tochemistry method．Results：The TNF- α serum level of endometriosis patients was significantly higher compared to the normal control(P<0.01)．The expression of NF-κB was major located in epithelioglandnlar kytoplasm of trusion-endometriosis，and the
expression of NF-κB during progestational stage was higher than that in proliferative stage (P<0．05). Conclusion：The abnormal expression of NF-κB and TNF-α were related to endometriosisB in the tissue of endometria and tumor necrosis factor- in serum in endometriosis.
摘要：
目的：检测核转录因子(NF—κB)在子宫内膜异位症(EM)患者异位内膜及在位内膜组织中的表达，以及其血清中肿瘤
坏死因子-α (TNF-α)的变化，探讨NF-κB与TNF-α在EM发病机制中的作用。方法：采用酶联免疫吸附试验(ELISA)测定TNF-α 的表达，免疫组化sP法检测NF-κB的表达情况。结果：子宫内膜异位症(EM)组血清TNF-α含量显著高于对照组(P<0．01)，且表达量随病情加重有上升趋势(P<0．05)。EM患者中异位内膜组NF-κB表达强度显著高于在位组及对照组(P<0．01)。结论：NF-κB与TNF-α在EM患者异位内膜中的异常高表达与EM的发病有密切关系。
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