枇杷叶膏中齐墩果酸和熊果酸的含量测定

J4 ›› 2011, Vol. 10 ›› Issue (12): 27-29.

枇杷叶膏中齐墩果酸和熊果酸的含量测定

目的：建立枇杷叶膏中齐墩果酸和熊果酸的反相高效液相色谱测定方法。方法：色谱柱：Agela venusil XBP-C18（250 mm×4.6 mm，5 μm）；流动相：甲醇- 0.05 mol/L 醋酸铵溶液（88:12）；流速为0.8 mL/min；检测波长为215 nm；柱温25 °C。结果：齐墩果酸在2.6 ~26 μg 范围内与峰面积有良好的线性关系，r=0.999 1；熊果酸在0.825~8.24 μg 范围内与峰面积有良好的线性关系，r=0.999 5；平均回收率分别为99.53%和99.37%，RSD 分别为0.28%和0.53%。结论：所用方法简单快速，专属性强，重现性好，可用于枇杷叶膏中齐墩果酸与熊果酸的含量测定。

大理学院药学与化学学院，云南大理 671000

收稿日期:2011-06-15 修回日期:2011-09-05 出版日期:2011-12-15 发布日期:2011-12-15
作者简介:苏丽娜，讲师，主要从事药物分析研究.
基金资助:
大理学院大学生科研基金项目（DXSKYX201019）

Content Determination for Oleanolic Acid and Ursolic Acid in Loquat Leaf Extract

School of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China

Received:2011-06-15 Revised:2011-09-05 Online:2011-12-15 Published:2011-12-15

摘要/Abstract

摘要：

关键词: 枇杷叶膏, 齐墩果酸, 熊果酸, 高效液相色谱

Abstract:

Objective: To establish a method for the measurement of oleanolic acid and ursolic acid in loquat leaf extract by RPHigh Performance liquid chromatography (RP-HPLC). Methods: With the RP-HPLC method, the chromatographic column was applied Agela technologies lnc XBP-venusil C18 (250 mm×4.6 mm, 5 μm); the mobile phase was applied methanol (0.05 mol/L) and ammonium acetate solution (88:12); the flow rate was 0.8 mL/min; and the detected wavelength was 215 nm, the column temperature was 25 °C. Results: The data showed a good linear relationship between peak area and concentration of oleanolic acid within 2.6-26
μg, r=0.999 1; and ursolic acid wintin 0.825-8.24 μg, r=0.999 5. The average recoveries were 99.53% and 99.37%, and RSD were 0.28% and 0.53%, respectively. Conclusion: The RP -HPLC method was simple, rapid, and with strong specificity and good reproducibility which can be applied in content determination for oleanolic acid and ursolic acid of Loquat leaf extract.

Key words: loquat leaf extract, oleanolic acid, ursolic acid, high performance liquid chromatography

中图分类号:

R284.2

苏丽娜, 董艳虹, 黄茂波, 李玉龙, 李雪峰. 枇杷叶膏中齐墩果酸和熊果酸的含量测定[J]. J4, 2011, 10(12): 27-29.

SU Li-Na, DONG Yan-Hong, HUANG Mao-Bo, LI Yu-Long, LI Xue-Feng. Content Determination for Oleanolic Acid and Ursolic Acid in Loquat Leaf Extract[J]. J4, 2011, 10(12): 27-29.

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