J4 ›› 2012, Vol. 11 ›› Issue (9): 98-100.
• 学生园地 • 上一篇
目的：建立小儿金翘颗粒中绿原酸的含量测定方法。方法：采用Agilent XBP-C18（4.6 mm×250 mm，5 μm）色谱柱，乙腈-0.4%磷酸（12:88）为流动相，流速1.0 mL/min，检测波长327 nm，柱温25 0C。结果：绿原酸在0.05~5.00 μg/mL之间有良好的线性关系（r =1.0），平均加样回收率100.44%，RSD为1.50%。结论：该法操作简便，准确，可靠，重现性好，可用于小儿金翘颗粒的质量控制。
Objective: To establish a HPLC method for content determination of chlorogenic acid in Xiaoerjinqiao Granules.
Methods: We applied the Agilent XBP-C18（4.6 mm×250 mm, 5μm）as the chromatographic column, and the mobile phase with acetonitrile-0.4% phosphoric acid solution（12:88）at the flow rate of 1.0 mL/min under the detection wavelength of 327 nm, and the column temperature of 25 0C. Results: There was a good linear relationship in the concentration range of 0.05~5.00 μg/mL （r =1.0）. The average recovery was 100.44% and RSD was 1.50%. Conclusion: The method appears to be simple, accurate and reliable, and can be used for the quality control of Xiaoerjinqiao Granules.
