双歧杆菌介导的福氏志贺菌Bb-ipaB疫苗的构建

J4 ›› 2013, Vol. 12 ›› Issue (3): 13-15.

双歧杆菌介导的福氏志贺菌Bb-ipaB疫苗的构建

大理学院基础医学院，云南大理 671000

收稿日期:2012-12-24 出版日期:2013-03-15 发布日期:2013-03-15
作者简介:王国富，副教授，主要从事细菌疫苗研究.
基金资助:
云南省教育厅基金重点项目基金资助项目（09Z0078）

Construction of the Recombinant Bb-ipaB Vaccine of Shigella flexneri

Pre-clinical College, Dali University，Dali, Yunnan 671000, China

Received:2012-12-24 Online:2013-03-15 Published:2013-03-15

摘要/Abstract

摘要：

目的：构建福氏志贺菌重组双歧杆菌Bb-ipaB疫苗。方法：以福氏志贺菌DNA为模板扩增福氏志贺菌ipaB基因，双酶切后
克隆到pGEX-1λT质粒上，构建重组质粒pGEX-ipaB，电穿孔法将该质粒转化入Bb，构建福氏志贺菌重组Bb-ipaB疫苗。结果：成功扩增出分子量约为1 711 bp的ipaB基因，双酶切证实基因成功插入pGEX-1λT中，并成功转化入双歧杆菌，构建rBb-ipaB疫苗。结论：成功构建福氏志贺菌重组rBb-ipaB疫苗，为该疫苗的进一步研究奠定了基础。

关键词: 福氏志贺菌, 双歧杆菌, 重组Bb-ipaB疫苗, 构建

Abstract:

Objective：To construct the recombinant Bb-ipaB vaccine of Shigella flexneri. Methods：ipaB gene was amplified by PCR and cloned into pGEX-1λT plasmid to construct pGEX-ipaB. The recombinant plasmid was electroporated into Bifidobacteria bifidum（Bb）to construct rBb-ipaB vaccine. Results：A 1 711 bp gene of ipaB was successfully amplified by PCR and cloned into pGEX-1λT by restriction analysis, and the rBb-ipaB vaccine was successfully constructed by PCR and restriction analysis. Conclusion：The rBbipaB vaccine of Shigella flexneri is successfully constructed, which lays the experimental foundation of exploitation and utilization of this vaccine.

Key words: Shigella flexneri, Bifidobacterium bifidum, recombinant Bb-ipaB vaccine, construction

中图分类号:

R378.2+5

王国富, 薛士鹏, 白丽, 吴利先. 双歧杆菌介导的福氏志贺菌Bb-ipaB疫苗的构建[J]. J4, 2013, 12(3): 13-15.

WANG Guo-Fu, XUE Shi-Peng, BAI Li, WU Li-Xian. Construction of the Recombinant Bb-ipaB Vaccine of Shigella flexneri[J]. J4, 2013, 12(3): 13-15.

参考文献

〔1〕Menard R，Dehio C，Sansonetti P J. Bacterial entery into
epithelial cells：the paradigm of Shigellosis〔J〕. Trends
Microbiol，1996，4（6）：220-226.
〔2〕Sasakawa C，Kamata K，Sakai T，et a1援Virulence-assosiated
genetic regions comprising 31 kilobases of the 230援
kilobaseplamaid in Shigella flexneri 2a 〔J〕援J Bacteriol，
1998，170：2480-2484援
〔3〕Menard R，Dehio C，Sansonetti P J. Bacterial entery into
epithelial cells；the paradigm of Shigellosis 〔J〕. Trends
Microbiol，1996，4：220-226.
〔4〕王国富，高峰，吴利先.幽门螺杆菌重组Bb-hpaA-vacA疫
苗的构建〔J〕.中国人兽共患病学报，2012，28（2）：131-134.
〔5〕Sani M，Botteaux A，Parsot C，et al. IpaD is localized at the
tip of the Shigella flexneri type III secretion apparatus〔J〕.
Biochim Biophys Acta，2007，1770（2）：307-311.
〔6〕Hamiaux C，Van E A，Parsot C，et al. Structural mimicry for
vinculin activation by IpaA，a virulence factor of Shigella
flexneri〔J〕. EMBO Rep，2006，7（8）：794-799.
〔7〕Ramarao N，Le C C，Izard T，et al. Capping of actin
filaments by vinculin activated by the Shigella IpaA
carboxyl-terminal domain〔J〕. FEBS Lett，2007，581（5）：
853-857.
〔8〕Sansonetti P J. Microbes and Microbial Toxin：Parardigms
for Microbial -Mucosal Interactins 芋Shigellosis：from
symptoms to molecular pathogenesis 〔J〕. Am J Physiol
Gastointest Liver Physiol，2001，280（3）：319-323.
〔9〕Lunelli M，Lokareddy R K，Zychlinsky A，et al. IpaB-IpgC
interaction defines binding motif for type III secretion
translocator 〔J〕. Biological Chemistry，2008，283：18646-
18654.
〔10〕Shen D K，Saurya S，Wagner C，et al. Domains of the
Shigella flexneri Type III Secretion System IpaB Protein
Involved in Secretion Regulation. American Society for
Microbiology〔J〕. Infect Immun，2010，78（12）：4999-5010.

[1]	董强，宋艳贺. 试论民族地区内涵式公共空间之美丽乡村构建[J]. J4, 2015, 14(9): 12-16.
[2]	高培仁, 张莉萍. 高师动物学教学中实施有效教学课堂策略探讨[J]. J4, 2014, 13(4): 81-84.
[3]	陈江丽，张嵘. 基于BP神经网络的教材质量评价研究[J]. J4, 2014, 13(12): 31-34.
[4]	和锡健，冯兆丽. 足球运动对构建纳西族和谐村落的作用[J]. J4, 2014, 13(12): 68-71.
[5]	牛坤, 吴建标. 大理地区医疗纠纷第三方调解机制构建研究[J]. J4, 2013, 12(5): 78-80.
[6]	. 福氏志贺菌ipaB基因的克隆及序列测定[J]. J4, 2012, 11(3): 28-30.
[7]	. 论我国刑事和解的理论基础[J]. J4, 2012, 11(1): 76-78.
[8]	. 人细胞周期蛋白cyclin E基因的克隆与真核表达载体的构建[J]. J4, 2011, 10(6): 21-24.
[9]	. 传播语言学理论的新构建 ———《传播语言学》评介[J]. J4, 2011, 10(3): 51-53.