J4 ›› 2013, Vol. 12 ›› Issue (3): 59-62.

• 临床医学 • 上一篇    下一篇

大理学院附属医院大肠埃希菌和肺炎克雷伯菌耐药性研究

  

  1. 1.大理学院基础医学院,云南大理 671000;2.大理学院附属医院,云南大理 671000
  • 收稿日期:2013-01-03 出版日期:2013-03-15 发布日期:2013-03-15
  • 作者简介:莫莉晓芳,硕士研究生,主要从事微生物与感染性疾病研究.
  • 基金资助:

    云南省科技厅基金资助项目(2010ZC146)

Analysis of Resistance of Escherichia Coli and Klebsiella Pneumoniae in Affiliated Hospital of Dali University

  1. 1.Pre-clinical College,Dali University,Dali,Yunnan 671000,China;
    2.Affiliated Hospital of Dali University, Dali, Yunnan 671000, China
  • Received:2013-01-03 Online:2013-03-15 Published:2013-03-15

摘要:

目的:对大理学院附属医院2011年至2012年临床分离的大肠埃希菌及肺炎克雷伯杆菌的耐药性进行分析,指导临床合
理应用抗生素。方法:菌株分离及培养按常规方法进行,细菌鉴定采用VITET-2 Compact 60全自动微生物分析仪,药敏试验采用K-B法。结果:共收集标本183株,其中大肠埃希菌146株:口痰92株(63%)、分泌物17株(11.6%)、尿液16株(10.9%),脓液9株(6.1%),其他12株(8.2%);肺炎克雷伯杆菌37株:口痰27株(73%)、分泌物5株(13.5%)、脓液3株(8%)其他2株(5.4%)。药敏试验结果显示:大肠埃希菌和肺炎克雷伯杆菌对头孢哌酮、阿米卡星、亚胺培南等耐药率较低,分别为10.3%、6.2%、2.7%和10.8%、13.5%、2.7%。结论:大肠埃希菌及肺炎克雷伯杆菌两年痰液中检出率呈上升趋势,对左氧氟沙星、头孢唑啉,氨曲南,头孢他啶等耐药率超过50%,特别是头孢他啶的耐药率相对于2008年显著升高,而对头孢哌酮、阿米卡星、亚胺培南等耐药性较低,临床应根据药敏实验合理选择抗生素。

关键词: 大肠埃希菌, 肺炎克雷伯杆菌, 耐药性分析

Abstract:

Objective: To instruct the clinical medicine in Affiliated Hospital of Dali University by analyzing the resistance of Escherichia coli and Klebsiella pneumoniae from 2011 to 2012. Methods: Isolate and culture strains by using conventional methods, and identify the bacteria with VITET-2 Compact 60 automatic microbial analyzer and test the susceptibility through K-B. Results: In total 183 specimens, 146 of them were Escherichia coli: koutan 92(63%), secretions 17(11.6%), urine 16(10.9%), pus 9(6.1% 2), other 12(8.2%); 37 of them were Klebsiella pneumoniae: koutan 27(73%), secretions five(13.5%), pus 3(8%), other susceptibility test results: the resistance rates of Cefoperazone, Amikacin, Imipenem were lower, at 10.3%, 6.2%, 2.7% and 10.8%, 13.5%, 2.7%, respectively. Conclusion: The detection rate of Escherichia coli and Klebsiella pneumoniae in sputum is rising in these two years, and the resistance rates have been exceeded more than 50% of levofloxacin, cefazolin, aztreonam, ceftazidime, especially ceftazidimewas resistance rates is significantly elevated. However, resistance rates of cefoperazone, amikacin, imipenem are lower, so doctors should choose antibiotics based on susceptibility experiments.

Key words: Escherichia coli, Klebsiella pneumoniae, resistance analysis

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