黑乳海参硫酸化多糖的提取纯化、结构特征及其抗凝活性

J4 ›› 2013, Vol. 12 ›› Issue (6): 12-16.

黑乳海参硫酸化多糖的提取纯化、结构特征及其抗凝活性

1.大理学院药学与化学学院，云南大理671000；
2.中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室，昆明650201

收稿日期:2013-03-18 修回日期:2013-04-06 出版日期:2013-06-15 发布日期:2013-06-15
作者简介:卢锋，硕士研究生，主要从事天然药物化学研究.
基金资助:
云南省高端人才引进计划基金资助项目（2010CI116）；
国家自然基金青年科学基金资助项目（81102372）

Extraction, Purification, Structure Characteristics and Anticoagulation of the Sulfated Polysaccharides from
the Sea Cucumber Holothuria nobilis

1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China; 2. State Key Laboratory of Phytochemistry and
Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China

Received:2013-03-18 Revised:2013-04-06 Online:2013-06-15 Published:2013-06-15

摘要/Abstract

摘要：

目的：研究黑乳海参（Holothuria nobilis Selenka）中硫酸化多糖类型、理化性质及抗凝活性。方法：利用化学和物理相结合
的分离方法进行纯化，采用色谱、化学、光谱等方法对其纯度、化学组成、分子量、特性黏度、旋光度、凝血活性等进行分析。结果：
分离获得F-I、F-II两种多糖组分。F-I由葡萄糖醛酸、乙酰氨基半乳糖、岩藻糖和硫酸基（摩尔比1.00:0.96:1.18:2.99）组成；F-II仅
由岩藻糖和硫酸基（摩尔比1.00:0.51）；它们的理化性质显著不同，前者的抗凝效价约为后者的6倍。结论：黑乳海参中至少存在
两种不同的硫酸化多糖，岩藻糖化糖胺聚糖和α-L-硫酸化岩藻聚糖，均具有强的抗凝血活性。

关键词: 海参, 多糖, 纯化, 理化性质, 抗凝活性

Abstract:

Objective: To study the polysaccharide types, physicochemical properties and anticoagulant activities of sulfated
polysaccharides from the sea cucumber Holothuria nobilis Selenka. Methods: Sulfated polysaccharides were purified by methods of
chemistry and physics. Physicochemical properties and biological activities of the saccharides, such as purity, chemical composition,
molecular weight, intrinsic viscosity, optical rotation and anticoagulant activities were determined by methods of chromatography,
chemistry, spectrum technologies, etc. Results: The polysaccharides were separated into two fractions, F-I and F-II, respectively. The
components of F-I were identified as glucuronic acid, aminogalactose, fucan and sulfate （molar ratio 1.00:0.96:1.18:2.99）; F-II
contained only fucan and sulfate （molar ratio 1.00:0.51）. The two polysaccharides were significantly different in the physicochemical
properties, and F-I had 6 times of anticoagulant potency compared to the F-II. Conclusion: The sea cucumber H. nobilis contains at
least two types of sulfated polysaccharides, a fucosylated glycosaminoglycan sulfate and a sulfated α-L -fucan which have
anticoagulant activities.

Key words: sea cucumber, polysaccharides, purification, physico chemical characteristics, anticoagulant activities

中图分类号:

R284.1

卢锋, 吴明一, 高娜, 刘光明, 赵金华. 黑乳海参硫酸化多糖的提取纯化、结构特征及其抗凝活性[J]. J4, 2013, 12(6): 12-16.

LU Feng, WU Ming-Yi, GAO Na, LIU Guang-Ming, ZHAO Jin-Hua. Extraction, Purification, Structure Characteristics and Anticoagulation of the Sulfated Polysaccharides from
the Sea Cucumber Holothuria nobilis[J]. J4, 2013, 12(6): 12-16.

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