J4 ›› 2013, Vol. 12 ›› Issue (6): 23-26.

• 药学 • 上一篇    下一篇

苦瓜酒中皂苷的含量测定

  

  1. 大理学院,云南大理671000
  • 收稿日期:2013-01-11 修回日期:2013-03-08 出版日期:2013-06-15 发布日期:2013-06-15
  • 作者简介:陈艳兰,教授,主要从事学位与研究生教育管理 研究.
  • 基金资助:

    大理学院应用开发研究基金资助项目(KYYY200904);
    大理市科技基金资助项目(DLKJ201001)

Content Determination of Momordicoside in Morrnordica charantia L.Wine

  1. Dali University, Dali, Yunnan 671000, China
  • Received:2013-01-11 Revised:2013-03-08 Online:2013-06-15 Published:2013-06-15

摘要:

目的:测定苦瓜酒中皂苷的含量。方法:以人参皂苷和茁-谷甾醇为对照品,香草醛-高氯酸分光度法测定皂苷含量。结
果:人参皂苷在0.030~0.240 mg范围内线性关系良好(r=0.999 1),平均加样回收率为99.6%,RSD=2.5%(n=6),样品含量在
0.151 1~0.563 0 mg;β-谷甾醇在0.015~0.116 mg范围内线性关系良好(r=0.999 3),平均加样回收率为100.9%,RSD=2.6%(n=6),
样品含量在0.065 6~0.247 9 mg。结论:β-谷甾醇和人参皂苷均可作为测定苦瓜皂苷含量的对照品,方法准确可靠,可用于苦瓜
酒的质量控制。

关键词: 紫外分光光度法, 苦瓜皂苷, 含量测定

Abstract:

Objective: To determine the content of the momordicoside in Morrnordica charantia L. wine. Methods: Vanillin -
perchloric acid colorimetry was applied to determine the contents of the total saponin in Morrnordica charantia L. wine, ginsenoside
and β-sitosterol were used as the reference substance. Results: The concentration of ginsenoside had good linearity in the range of
0.030-0.240 mg(r=0.999 1). The average recovery rate was 99.6%, RSD=2.5%(n=6), the content of sample was 0.151 1-0.563 0 mg.
The concentration of 茁-sitosterol had good linearity in the range of 0.015-0.116 mg(r=0.999 3). The average recovery was 100.9%,
RSD= 2.6% (n=6), the content of sample was 0.065 6-0.247 9 mg. Conclusion: β-sitosterol and ginsenoside are suitable for
reference substance to determine the content of saponin in Morrnordica charantia L. wine. The method was accurate which could be
used for the quality control of Morrnordica charantia L.Wine.

Key words: UV, momordicoside, contents determination

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