单核细胞增生李斯特菌三种检测方法的比较

J4 ›› 2013, Vol. 12 ›› Issue (6): 38-42.

单核细胞增生李斯特菌三种检测方法的比较

昆明市疾病预防控制中心，昆明 650228

收稿日期:2012-09-16 修回日期:2012-10-16 出版日期:2013-06-15 发布日期:2013-06-15
作者简介:张烜榕，初级检验师，主要从事病原生物检验研究.

Comparison of Three Methods of Listeria monocytogenes Detection

Disease Control and Prevention Center of Kunming, Kunming 650228, China

Received:2012-09-16 Revised:2012-10-16 Online:2013-06-15 Published:2013-06-15

摘要/Abstract

摘要：

目的：通过比较国标法、real-time PCR法和LAMP方法，得到适合基层实验室快速检测单核细胞增生李斯特菌的方法。方
法：分别用国标法、real-time PCR法和环介导的等温扩增（loop-mediated isothermal amplification，LAMP）法检测李斯特菌，并进行方法比较。结果：三种方法对单增李斯特菌的检测结果一致.国标法整个检测过程需5~7 d；real-time PCR法实验条件要求高，成本高，检测需1.5~2.5 d；LAMP法实验条件低，成本低，检测时限与real-time PCR相同。结论：LAMP法检测单增李斯特菌灵敏度高、特异性强、快速高效和低成本，适合基层实验室应急检测和现场监测使用。

关键词: 国标法, LAMP, PCR, 单核细胞增生李斯特菌检测

Abstract:

Objective: To find a suitable method for rapid detection of Listeria monocytogenes （LM）in grassroots labs. Methods:
GB law, real-time PCR and LAMP were adopted to detect LM, then, the results were evaluated. Results: The results of the three mothods were quite consistent. The detection process of GB law took 5 to 7 days. While the real -time PCR requiring high experimental conditions and cost took 2.5 days. LAMP method, requiring low experimental conditions and cost, took the same time as the real-time PCR. Conclusion: The LAMP assay was a sensitive, specific, rapid and economical method for the detection of LM, which could be widely used by grassroots labs.

Key words: GB law, LAMP, PCR, detection of Listeria monocytogenes

中图分类号:

R155.5

张烜榕, 颜军, 李文龙, 侯敏, 蒋玉佳, 卜舒, 李桂满. 单核细胞增生李斯特菌三种检测方法的比较[J]. J4, 2013, 12(6): 38-42.

ZHANG Xuan-Rong, YAN Jun, LI Wen-Long, HOU Min, JIANG Yu-Jia, BO Shu, LI Gui-Man. Comparison of Three Methods of Listeria monocytogenes Detection[J]. J4, 2013, 12(6): 38-42.

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