不同产地不同采收期岩陀药材中槲皮素的含量测定

J4 ›› 2013, Vol. 12 ›› Issue (9): 21-24.

不同产地不同采收期岩陀药材中槲皮素的含量测定

1.大理州中医医院，云南大理 671000；2.大理学院药学与化学学院，云南大理 671000

收稿日期:2013-07-02 修回日期:2013-07-15 出版日期:2013-09-15 发布日期:2013-09-15
作者简介:金杨，主管药师，主要从事新药开发、合理用药研究.
基金资助:
大理学院青年教师基金资助项目（KY201136）

Determination of Quercetin in Rhizoma Rodgersiae from Different Areas and Different Harvest Time

1. Chinese Traditional Medicine Hospital of Dali Prefecture, Dali, Yunnan 671000, China;
2. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China

Received:2013-07-02 Revised:2013-07-15 Online:2013-09-15 Published:2013-09-15

摘要/Abstract

摘要：

目的：测定不同产地不同采收期岩陀药材中槲皮素的含量。方法：采用HPLC法测定岩陀药材中槲皮素的含量。流动相甲
醇-0.5%磷酸溶液（50:50），检测波长370 nm，流速1.0 mL/min，柱温30⁰C。结果：岩陀药材中槲皮素含量差异较大，不同产地同一采收期的含量范围为0.129%~0.207%；不同采收期同一产地的含量范围为0.074%~0.129%。结论：该方法操作简便，结果准确，重复性好，可为该药材合理开发利用提供科学依据。

关键词: 岩陀, 槲皮素, HPLC, 含量测定

Abstract:

Objective: To establish a method for the determination of Quercetin in Rhizoma Rodgersiae from different areas and
different harvest time. Methods: The contents of Quercetin were determined by HPLC. The mobile phase consisted of methanol-0.5% phosphoric acid （50:50）, the detection wavelength was 370 nm, with the flow rate of 1.0 mL/min and the temperature at 30 ⁰C.Results: The contents of Quercetin in Rhizoma Rodgersiae from different areas and different harvest time varied greatly. The contents of Quercetin in Rhizoma Rodgersiae from different areas with the same harvest time were 0.129%-0.207%. The contents of Quercetin in Rhizoma Rodgersiae from different harvest time with the same areas were 0.074%-0.129% . Conclusion: The method for determination of Quercetin is simple, accurate and with good repeatability, which could provide a scientific basis for rational development and utilization.

Key words: Rhizoma Rodgersiae, Quercetin, HPLC, content determination

中图分类号:

R284

金杨, 王燕. 不同产地不同采收期岩陀药材中槲皮素的含量测定[J]. J4, 2013, 12(9): 21-24.

JIN Yang, WANG Yan. Determination of Quercetin in Rhizoma Rodgersiae from Different Areas and Different Harvest Time[J]. J4, 2013, 12(9): 21-24.

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