蚕豆根尖微核试验研究云南松松塔球提取物抑突变性

J4 ›› 2014, Vol. 13 ›› Issue (2): 55-57.

蚕豆根尖微核试验研究云南松松塔球提取物抑突变性

1.大理学院基础医学院，云南大理 671000；2.大理学院农学与生物科学学院，云南大理 671003；3.大理学院药学与化学学院，云南大理671000；4.大理学院公共卫生学院，云南大理 671000；5.南华大学公共卫生学院，湖南衡阳 421001

收稿日期:2013-09-26 修回日期:2013-12-19 出版日期:2014-02-15 发布日期:2014-02-15
作者简介:林牧，硕士研究生，主要从事病毒感染与免疫研究.

Anti mutagenicity of Extracts of the Yunnan Pinecones by Vicia-Micronucleus Test

（1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China；2. College of Agriculture and Biology Science, Dali University, Dali, Yunnan 671003, China；3. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China；4. College of Public Health, Dali University, Dali, Yunnan 671000, China；5. School of Public Health, University of South China, Hengyang, Hunan 421001, China）

Received:2013-09-26 Revised:2013-12-19 Online:2014-02-15 Published:2014-02-15

摘要/Abstract

摘要：

目的：探讨云南松松塔球提取物的抑突变作用。方法：分别将大理邓川和湾桥产的蚕豆随机分为4组，经实验处理后，
采用蚕豆根尖微核技术对蚕豆根尖细胞微核数进行检测，考察松塔球提取物对蚕豆根尖细胞微核率的影响情况。结果：松塔球提取物I对邓川产蚕豆根尖细胞微核的产生有抑制作用，抑制率达50%，且对环磷酰胺诱导的蚕豆根尖微核有明显的抑制作用，抑制率为62.5%。松塔球提取物I对湾桥产蚕豆根尖细胞微核的产生有抑制作用，抑制率达50%，且对环磷酰胺诱导的蚕豆根尖细胞微核有明显的抑制作用，抑制率为50%。结论：松塔球提取物可有效抑制环磷酰胺诱导的蚕豆根尖微核的产生，具有抑突变性，从而说明松塔球提取物对肿瘤的防治可能具有积极的作用。

关键词: 松塔球提取物, 抑突变性, 蚕豆根尖微核技术

Abstract:

Objective: To investigate the anti- mutagenicity of the extracts of Yunnan pincones. Methods: Horsebeans from
Dengchuan village and Wanqiao village were divided into 4 groups randomly. After the pretreatment, vicia-micronucleus test was applied to explore the anti- mutagenicity of the extracts of pinecones. Results: The extract I of Yunnan pinecone showed 50% inhibition on the Dengchuan fava beans-induced micronucleus, 62.5% inhibition on cyclophosphamide-induced micronucleus and 50% inhibition on Wanqiao fava beans root-induced micronucleus. Conclusion: Yunnan pinecone extract effectively inhibited viciamicronucleus induced by cyclophosphamide solution and showed anti-mutagenicity. Thus the extracts of pinecones may have positive effect on tumor prevention.

Key words: pinecones extract, anti-mutagenicity, Vica-micronucleus test

中图分类号:

B849

林牧, 刘光明, 蒋锡超, 李云静, 马志敏. 蚕豆根尖微核试验研究云南松松塔球提取物抑突变性[J]. J4, 2014, 13(2): 55-57.

LIN Mu, LIU Guang-Ming, JIANG Xi-Chao, LI Yun-Jing, MA Zhi-Min. Anti mutagenicity of Extracts of the Yunnan Pinecones by Vicia-Micronucleus Test[J]. J4, 2014, 13(2): 55-57.