J4 ›› 2014, Vol. 13 ›› Issue (4): 54-55.

• 临床医学 • 上一篇    下一篇

大理某医院检验报告单带菌情况分析

  

  1. 五华区人民医院,昆明 650031
  • 收稿日期:2014-01-03 修回日期:2014-01-28 出版日期:2014-04-15 发布日期:2014-04-15
  • 作者简介:董立文,主要从事细菌的耐药性及耐药机制研究.

Investigation on the Bacterial Contamination of Laboratory Sheets in a Hospital of Dali

  1. Wuhua District People's Hospital,Kunming 650031,China
  • Received:2014-01-03 Revised:2014-01-28 Online:2014-04-15 Published:2014-04-15

摘要:

目的:研究检验报告单的带菌情况,并行菌属及菌种类型分析。方法:在检验报告单上直接取样增菌接种于血琼脂平板
和麦康凯平板培养,用形态学检验及生化反应鉴定到种。结果:1 000份检验报告单,血琼脂平板共生长细菌719株,其中葡萄球菌属518株占72.04%,肠道杆菌175株占24.34%,枯草杆菌26株占3.62%。麦康凯平板共生长细菌369株,其中葡萄球菌属299株占81.03%,肠道杆菌70株占18.97%。结论:检验报告单主要带菌为葡萄球菌和肠道杆菌,其中葡萄球菌以表皮葡萄球菌和金黄色葡萄球菌为主,肠道杆菌以耶氏菌和志贺氏菌为主。

关键词: 检验报告单, 带菌情况, 菌种类型

Abstract:

Objective:To investigate the species of bacterial contamination of laboratory sheets. Methods:laboratory sheets were direct sampled and enrichment culture then inoculating blood Columbia agar plates and MacConkey Agar plates. Isolated bacteria were identified by morphology,Gram stain and biochemical test. Results:From 1 000 laboratory sheets,after inoculate,there were 719 bacteria growing in blood agar platein. The differential strains grew on blood Columbia agar plates and their infection rates were:Staphylococcus,518 strains,infection rates was 72.04%;Enteric bacilli,175 strains,infection rates was 24. 34%;Bacillus subtilis,26 strains,infection rates was 3.62%. The differential strains grew on MacConkey Agar plates and their infection rates were :Staphylococcus,229 strains,infection rates was 81.03%;Enteric bacilli,70 strains,infection rates was 18.97%. Conclusion:In the inspection report,the main bacteria are mainly including Staphylococcus and Enterobacteriaceae. Staphylococcus is mainly Staphylococcus epidermidis or Staphylococcus aureus,and Enterobacteriaceae is mainly Yersinia or Shigella.

Key words: laboratory sheets, bacterial contamination, species

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