结核分枝杆菌MTB8.1蛋白自诱导发酵研究

J4 ›› 2014, Vol. 13 ›› Issue (8): 18-22.

结核分枝杆菌MTB8.1蛋白自诱导发酵研究

1.大理学院基础医学院，云南大理 671000；2.北京四环科宝制药有限公司，北京 100070

收稿日期:2014-02-24 出版日期:2014-08-15 发布日期:2014-08-15
作者简介:聂恒，硕士研究生，主要从事感染与免疫学研究.
基金资助:
国家自然科学基金资助项目（81260456）

The Study of Auto-inducing Fermentation of Mycobacterium tuberculosis Protein MTB8.1

1. Pre-clinical College, Dali University, Dali,Yunnan 671000,China; 2. SHKB Pharmaceutical Co., Ltd., Beijing 100070, China

Received:2014-02-24 Online:2014-08-15 Published:2014-08-15

摘要/Abstract

摘要：

目的：初步摸索出一条适合工业化开发、高效的、可溶表达结核分枝杆菌MTB8.1蛋白的发酵工艺。方法：利用菌株
pUC18/MTB8.1/DH10B为发酵株，通过对培养基组成和发酵参数进行系统研究，从而获得一全新的发酵工艺。结果：重组结核分枝杆菌MTB8.1蛋白通过本发酵工艺的表达，单位体积发酵液可溶性蛋白的产量大幅提高，每升发酵液纯化得到可溶性蛋白约30 mg左右。结论：初步摸索出一条较好的MTB8.1在大肠埃希菌系统中的可溶性表达的途径，为其工业化生产奠定了理论基础。

关键词: 结核分枝杆菌, MTB8.1蛋白, 可溶性表达, 发酵工艺, 抗原性

Abstract:

Objective: To explore an effective fermentation process of Mycobacterium tuberculosis protein MTB8.1 with soluble
expression, which would be suitable for industrial development. Methods: Strain pUC18/MTB8.1/DH10B was used as fermentation strain and a systematic study of the composition of medium and the parameters of fermentation was conducted to obtain a new fermentation process. Results: By means of the new fermentation process, the yield of soluble recombinant protein MTB8.1 was greatly increased in per unit volume of fermentation broth, with about 30 mg of soluble protein obtained from every liter of fermentation broth through purification. Conclusion: A better way of soluble expression of MTB8.1 in Escherichia coli expression system was found tentatively, which laid the theoretical foundation for the industrial production.

Key words: Mycobacterium tuberculosis, protein MTB8.1, soluble expression, fermentation process, antigenicity

中图分类号:

聂恒, 高志芳, 吴利先. 结核分枝杆菌MTB8.1蛋白自诱导发酵研究[J]. J4, 2014, 13(8): 18-22.

NIE Heng, GAO Zhi-Fang, WU Li-Xian. The Study of Auto-inducing Fermentation of Mycobacterium tuberculosis Protein MTB8.1[J]. J4, 2014, 13(8): 18-22.

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