J4 ›› 2014, Vol. 13 ›› Issue (10): 14-17.

• 药学 • 上一篇    下一篇

紫地榆提取物体外抗菌活性研究

  

  1. 1.大理学院基础医学院,云南大理671000;2.大理学院药学与化学学院,云南大理671000;
    3.楚雄医药高等专科学校,云南楚雄675000
  • 收稿日期:2014-06-26 出版日期:2014-10-15 发布日期:2014-10-15
  • 作者简介:耿玲,助理实验师,主要从事病原微生物学研究.

The Antibacterial Activity of Extracts from Geranium strictipes R. Kunth in Vitro

  1. 1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China; 2. College of Pharmacy and Chemistry, Dali University, Dali,
    Yunnan 671000, China; 3. Chuxiong Pharmaceutical College, Chuxiong, Yunnan 675000, China
  • Received:2014-06-26 Online:2014-10-15 Published:2014-10-15

摘要:

目的:从紫地榆乙酸乙酯部位分离得到不同组分,并对其进行体外抗菌活性研究。方法:通过大孔树脂柱对紫地榆乙
酸乙酯部位分离,分别用水和不同浓度的乙醇进行梯度洗脱,不同极性提取物用常量试管稀释法对13株常见病原菌和10株耐
甲氧西林金黄色葡萄球菌进行抑菌作用研究。结果:水部位提取物对标准菌株无明显抑菌活性;50%乙醇提取物对13株常见
病原菌和10株临床分离的MRSA均有不同程度的抑菌作用。结论:紫地榆50%乙醇提取物在体外有不同程度的抑菌活性。

关键词: 紫地榆, 体外抗菌活性, 最低抑菌浓度, 耐甲氧西林金黄色葡萄球菌

Abstract:

Objective: To study the antibacterial activity in vitro of different extracts isolated from the part of ethyl acetate extracted
from Geranium strictipes R. Kunth. Methods: The ethyl acetate extracts were eluted by H2O and the different concentration of the
ethanol through the macro-porous resin column, then the antibacterial effects of different extracts on the 13 common pathogenic
bacteria and MRSA were conducted by the method of constant tube dilution. Results: Water extract had no obviously antibacterial
activity on the standard bacterial strains. 50% ethanol extracts had a certain antibacterial activity at different degree on 13 common
pathogenic bacteria and 10 MRSA. Conclusion: The 50% ethanol extracts from Geranium strictipes R. Kunth have significant
antibacterial effect in vitro.

Key words: Geranium strictipes R. Kunth, in vitro antibacterial activity, minimum inhibitory concentration, Methicillin resistant
Staphylococcus aureus

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