J4 ›› 2014, Vol. 13 ›› Issue (12): 48-51.

• 生命科学 • 上一篇    下一篇

红瘰疣螈外周血细胞组织学观察

  

  1. 大理学院农学与生物科学学院,云南大理671003
  • 收稿日期:2014-01-18 出版日期:2014-12-15 发布日期:2014-12-15
  • 作者简介:宋绍萍,生物科学专业2008级本科生.
  • 基金资助:

    云南省高校滇西生物多样性科研创新团队项目
    (云教函〔2011〕93号);大理学院质量工程项目
    (TDI-05);大理学院应用开发基金资助项目
    (KYYY201002)

Hematological Observation of Tylototriton shanjing

  1. College of Agriculture and Biology Science, Dali University, Dali, Yunnan 671003, China
  • Received:2014-01-18 Online:2014-12-15 Published:2014-12-15

摘要:

以采自大理点苍山的红瘰疣螈为材料,应用常规血涂片,Giemsa染色法与血细胞计数法观察并统计红瘰疣螈外周血细胞
的形态特征及其数量比例。结果表明:红瘰疣螈的外周血细胞中红细胞有核,呈椭圆形、梨形,平均含量为1.48×105 个/mm3 。白
细胞多呈近圆形,平均含量为0.17×105 个/mm3。白细胞中按细胞数量从多到少排列,其顺序依次为淋巴细胞、嗜中性粒细胞、
嗜酸性粒细胞、单核细胞和嗜碱性粒细胞。并将研究结果与其他动物进行了比较,从进化、生活习性、环境与血细胞含量之间
的关系进行了讨论。

关键词: 红瘰疣螈, 血细胞, 组织学

Abstract:

The study observes morphological features and counts numbers of peripheral blood cells of Tylototriton shanjing from
Cangshan Mountain by routine blood smears, Wright-Giemsa stain and hemacytometry.The results show that erythrocytes in peripheral
blood cells have nucleus, elliptic or pear-shaped, with a mean density of 1.48×105/mm3, and most leucocytes are round, with a mean
density of 0.17×105/mm3. Lymphocytes are the most numerous in leucocytes, followed in order by neutrophils, eosinophils, monocytes
and basophils. The results are also compared with other vertebrates and the relationship between the number of blood cells and
evolution, life habits and environment is discussed.

Key words: Tylototriton shanjing, blood cells, histology

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