HPLC Method for Determination of the Inosine Content in Ganlong Capsule

Abstract

Abstract:

Objective: To establish a determination method of the content of inosine in Ganlong capsule for providing the basis for
Ganlong capsule quality control. Methods: Gemini 5u C18 110A（250 mm×4.6 mm）chromatographic column, mobile phase water
（0.05%TFA）: acetonitrile（0.05% TFA）, gradient elution, 0.8 mL/min flow, 30 ℃ column temperature, DAD detector, 214 nm detection
wavelength, 10 μL sample quantity were used in the study. Results: Peak area of inosine between 0.202-2.020 μg range and injection
volume（μg）showed a linear relationship. The average recoveries（n=9）was 96.9% , RSD=2.18% . Conclusion: The established
method of determination Ganlong capsules is scientific, simple and accurate, which can be used to improve Ganlong capsule quality
standard test project.

Key words: Ganlong capsule, inosine, determination

CLC Number:

R917

Li Yuchi, Xu Yan, Yang Yongshou, Xiao Peiyun. HPLC Method for Determination of the Inosine Content in Ganlong Capsule[J]. , 2016, 1(12): 40-42.

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HPLC Method for Determination of the Inosine Content in Ganlong Capsule

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