Abstract: Objective: To explore the clinical and pathological features of occult hepatitis B in Yunnan Province. Methods: Serum
HBV（Hepatitis B Virus, HBV）DNA levels were tested in 5 000 HBsAg negative patients. Clinical, histopathologic and immunohistologic
tests were done in 26 HBV DNA positive, occult hepatitis B patients. Results: Histopathologically, 26 cases were chronic hepatitis,
among them, 24 had mild to moderate inflammation, 2 had severe inflammation. 25 had mild to moderate fibrosis, 1 had early cirrhosis.
There were 2 cases of mild fibrosis, 17 cases of moderate fibrosis, 6 cases of severe fibrosis, 1 case of early cirrhosis according to
Fibroscan tests. The Fibroscan results coincided with the pathologic results. Immunohistologically, 9 cases were HBsAg positive, 10
were HBcAg positive. Conclusion: Occult hepatitis B is one of the main cause of end-stage occult liver diseases and needs to be
diagnosed and treated immediately.

Key words: occult hepatitis B, HBV DNA, qPCR, histopathology examination, immunochemistry