Isolation and Identification of Lactic Acid Bacteria with Strong Inhibitory Effects Against Enterobacter cloacae

doi:10. 3969/j. issn. 2096-2266. 2021. 10. 006

Abstract

Abstract:

Objective：To isolate and screen out lactic acid bacteria strains with inhibitory advantages against Enterobacter cloacae from pickles in Dali， and to enrich the potential weight loss function of traditional lactic acid fermented foods. Methods： Pickle samples were collected in Dali area， cultured and separated by BBL agar medium， Gram staining， PCR identification， and antibacterial activity analysis； strips were cut gel， mass amplified， TA clones constructed， and sequenced. The obtained gene sequence information was compared with the Blast data in NCBI to find the bacteria containing this band of information. Results： Among the 18 samples， the candidate strain with the best inhibitory effect was isolated. Through comparing the sequencing results， it was clear that it was Lactobacillus plantarum. Conclusion： A strain of lactic acid bacteria with high inhibitory effect on the standard strain of Enterobacter cloacae ATCC 700323 was successfully isolated.

Key words:

"> pickle">； isolation of lactic acid bacteria">； inhibition of Enterobacter cloacae">； colony PCR

CLC Number:

TS201.3

Li Yafei, Luo Chunyu, Liu Qi.

Isolation and Identification of Lactic Acid Bacteria with Strong Inhibitory Effects Against Enterobacter cloacae [J]. Journal of Dali University, 2021, 6(10): 32-.

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