Study on Optimal
Extraction Duration of Soil-Nematode Based on Baermann Funnel Method

Abstract

Abstract:

The Baermann funnel method is common in soil-nematode extraction. However， it has been found that the extraction durations in studies varies greatly， and there is a lack of agreed standard. To explore the optimal extraction duration， the Baermann funnel method was used to extract soil-nematode for 72 h， and the cumulative separation rates were calculated for 12， 24， 36， 48，60 and 72 h respectively. The results showed that in terms of the separation of taxa， 24 h was the peak value， and it has no difference from that of 48 h（21.00±2.54 vs. 21.00±1.00，P=0.817）； in terms of the separation of numbers， 36 h was the most appropriate time， and the cumulative separation rate was 99.63%. A total of 24 777 nematodes were isolated in the experiment， belonging to 2 classes， 6 orders， 21 families and 35 genera， covering a large number of nematode groups and having universal applicability. Considering the taxa and numbers， the recommended separation time of the Bellman funnel method is 36 h.

Key words:

"> , soil-nematode, Baernrmann funnel method, extraction duration

CLC Number:

Q939.96

Pu Guoqing, Luo Dan, Zhao Chengfa, Tan Kun, Liu Shuoran, Xiao Wen, Li Yanpeng.

Study on Optimal Extraction Duration of Soil-Nematode Based on Baermann Funnel Method [J]. Journal of Dali University, 2021, 6(12): 87-90.

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Study on Optimal Extraction Duration of Soil-Nematode Based on Baermann Funnel Method

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