Preparation and Cytotoxicity of Dextran Particles of Polypeptide from Periplaneta americana

Abstract

Abstract:

〔Abstract〕 Objective： To study the preparation and cytotoxicity of dextran particles of polypeptide from Periplaneta americana in vitro. Methods： The dextran particles of polypeptide from Periplaneta americana were prepared by low temperature induced phase separation. The particles were observed by optical microscopy and scanning electron microscopy. In vitro cytotoxicity of dextran particles of polypeptide from Periplaneta americana was detected by real-time unmarked cell analysis technique and flow cytometry. Based on this， its biosafety was evaluated.Results： The dextran particles of polypeptide from Periplaneta americana were round and uniform with an average particle size of 5 μm， drug loading of 86.70% and the encapsulation rate of 66.70%. When the concentration of dextran particles of polypeptide from Periplaneta americana was 0.00， 0.05， 0.10， 0.20， 0.40 mg/mL， the survival rates of HepG2 cells were 97.76%， 86.44%， 87.20%， 83.48% and 83.23%， respectively， after being treated for 24 hours. It can be seen that the dextran particles of polypeptide from Periplaneta americana didn't display potent cytotoxicity against HepG2 cells. Conclusion： In this study， the dextran particles of polypeptide from Periplaneta americana were prepared with no obvious cytotoxicity and can be further applied to the research of powder inhalation or sustained-release microspheres.

Key words:

"> font-size:10.5pt, ">polypeptide from Periplaneta americana, dextran particles, cytotoxicity

CLC Number:

Gao Ran, Zhang Weiwei, Ji Chunyan, Liu Guangming, Guo Meixian, Liu Xiaobo.

Preparation and Cytotoxicity of Dextran Particles of Polypeptide from Periplaneta americana [J]. Journal of Dali University, 2022, 7(10): 5-10.

References

〔1〕孙仁凇，张建斌，房佳霓，等.壳聚糖纳米粒作为药物递送系统在癌症治疗中的应用〔J〕.中国临床药理学与治疗学，2021，26（1）：65-75.

〔2〕石秀江.控释缓释微球制剂在肿瘤治疗中的研究进展〔J〕.生物化工，2021，7（3）：160-163.

〔3〕杨英来，杨文卿，武洋，等. β-葡聚糖对S180荷瘤小鼠实体瘤抑制作用及其机制研究〔J〕.新疆医科大学学报，2021，44（8）：953-957.

〔4〕王钦，刘克娜，孔彩华，等.美洲大蠊有效成分的提取及药理活性研究进展〔J〕.中华中医药学刊，2021，39（8）：108-111.

〔5〕耿巧玉.美洲大蠊活性部位提取分离工艺优化与初步抗肿瘤作用研究〔D〕.济南：山东中医药大学，2019.

〔6〕张鸿瀚，张蕊，王瑶，等.美洲大蠊多肽PAP-2对肝癌血管生成、侵袭及转移能力的影响〔J〕.大理大学学报，2021，6（2）：1-9.

〔7〕吕鸿，王瑶，张蕊，等.美洲大蠊多肽PAP-2对H22荷瘤小鼠的抑瘤作用研究〔J〕.中国药房，2019，30（7）：927-931.

〔8〕 NIE T Q，WANG W，LIU X H，et al. Sustained Release Systems for Delivery of Therapeutic Peptide/Protein〔J〕. Biomacromolecules，2021，22（6）：2299-2324.

〔9〕 HU Q B，LU Y J，LUO Y C. Recent Advances in Dextran-Based Drug Delivery Systems： From Fabrication Strategies to Applications〔J〕. Carbohydr Polym，2021，264：117999.

〔10〕 KRAISOMDET P，PRATESS T，NA NAKORN P，et al.

Amphiphilic Dextran-Vinyl Laurate-Based Nanoparticles：Formation， Characterization， Encapsulation， and Cytotoxicity on Human Intestinal Cell Line〔J〕. Prog Biomater，

2020，9（1/2）：15-23.

〔11〕徐云，张昆明，黄永春，等.孔板空化强化制备京尼平-壳聚糖载药纳米微球的工艺研究〔J〕.广西科技大学学报，2021，32（4）：9-16.

〔12〕 ZHOU H M，TONG H，LU J，et al. Preparation of Bio-Based Cellulose Acetate/Chitosan Composite Film with Oxygen and Water Resistant Properties〔J〕.Carbohydr Polym，2021，270：118381.

〔13〕赵琢，柳明，杨磊，等.多壁碳纳米管致人肝癌细胞HepG2毒性及代谢酶表达变化〔J〕.新型炭材料，2019，34（6）：600-605.

〔14〕 OBERG H H， PETERS C， KABELITZ D， et al. Real-Time Cell Analysis（RTCA） to Measure Killer Cell Activity against Adherent Tumor Cells in vitro〔J〕. Methods Enzymol，2020，631：429-441.

〔15〕李艳伟，宋兴辉，王佳佳，等.实时无标记肿瘤细胞凋亡筛选技术体系的建立〔J〕.生物技术通报，2019，35（10）：220-226.

〔16〕王景，曹广超，蒋光慧，等.蛇床子素对宫颈癌Hela-S3细胞活性、凋亡及相关信号通路的影响〔J〕.中国免疫学杂志，2020，36（23）：2850-2854.

〔17〕张宇轩.美洲大蠊提取物对肺腺癌A549细胞凋亡及PI3K/AKT信号通路的影响〔D〕.郑州：郑州大学，2019.

〔18〕庞丽然，贺丞，魏敬双，等.蛋白多肽类药物长效化技术研究策略〔J〕.生物技术进展，2021，11（3）：304-310.

〔19〕 SU Y，ZHANG B，SUN R W，et al. PLGA-Based Biodegradable Microspheres in Drug Delivery： Recent Advances in Research and Application〔J〕. Drug Deliv，2021，28（1）：1397-1418.

〔20〕万妮，陈斌，李合，等.肺部吸入给药系统的研究进展〔J〕.中国新药杂志，2021，30（15）：1386-1395.

[1]	Xie Diyang, Peng Zhijie. On the Extraterritorial Jurisdiction in the International Environmental Civil Public Interest Litigation [J]. Journal of Dali University, 2022, 7(7): 46-55.
[2]	Zhang Huan. The Dilemma and Outlet of Cambodian Chinese Education under the Background of Belt and Road Initiative [J]. Journal of Dali University, 2022, 7(7): 104-108.
[3]	Ou Ling, Li Cailin, Li Yinrui, Zhou Jie, Shan Wanxin, Wu Dingyu, Peng Fang. In vitro Study on the Periplaneta americana Polypepticde PAE2 Reversing Multi-Drug Resistance of BEL-7402/5-FU Based on AMPK-Regulated Autophagy [J]. Journal of Dali University, 2022, 7(4): 1-7.
[4]	Qing Chen, Chen Jinghu, Li Bolong, Liu Heng, Zhang Chenggui. Comparative Analysis of Liposoluble Constituents in the Larvae and Adults of Black Soldier Fly by GC-MS [J]. Journal of Dali University, 2022, 7(4): 8-11.
[5]	Wang Ruihang, Liu Xiaobo, Liao Ｓi, Hua Peng. Meta-Analysis of Cutaneous Adverse Reactions Induced by Dabrafenib Combined with Trametinib in the Treatment of Melanoma [J]. Journal of Dali University, 2022, 7(4): 12-20.
[6]	Hong Wenjuan, Zou Jiulin, Yu Zhuorui, Cheng Jiamao, He Minhua. Effects of SGLT2i on Bone Metabolism in Nephrotic Syndrome Model Rats [J]. Journal of Dali University, 2022, 7(4): 21-25.
[7]	Li Bin, Li Sufen, Yang Na, Xiong Wei. Expression and Significance of THBS2 Gene mRNA in Human Malignant Pleural Mesothelioma Based on Tumor Databases [J]. Journal of Dali University, 2022, 7(4): 26-33.
[8]	Duan Yi, Ni Changwen, Wang Minhua, Sun Sujiao, Huang Ling. Effects of the 7-Hydroxyisoflavone in the Proliferative Activity of HaCaT Cells and UVB-induced Photodamage [J]. Journal of Dali University, 2022, 7(4): 34-39.
[9]	Huang Zhong, Jia Ruipeng, Liu Yiheng, Zhang Chao, Liu Ke. Analysis of Risk Factors for Bone Cement Leakage during Percutaneous Kyphoplasty [J]. Journal of Dali University, 2022, 7(4): 40-43.
[10]	Luo Xinlei, Zhao Hui, Bai Tao, Xu Song, Zhang Xiongwen, Na Qiang. Analysis of Influencing Factors of New Fractures after Osteoporotic Vertebral Compression Fracture Surgery [J]. Journal of Dali University, 2022, 7(4): 44-46.
[11]	Xu Sanxiong, Zhang Jiyuan, Ma Xueying, Wei Guosong, Li Lin. Comparison of Curative Effects of MIS-TLIF and PLIF in the Treatment of Single-Segment Lumbar Degenerative Diseases [J]. Journal of Dali University, 2022, 7(4): 47-51.
[12]	Liu Siyuan, Rao Tao, Zhao Heng, Dou Jichen, Zhou Yi, Liu Qinghua, Cun Ｘinhua, . Clinical Evaluation of Oblique Lateral Interbody Fusion in the Treatment of Elderly Patients with Lumbar Tuberculosis [J]. Journal of Dali University, 2022, 7(4): 52-56.
[13]	Hu Jianhua, Hu Qi, Gao Guoxi, Lian Xingye, Yang Zhao, Zhang Chao. Analysis of the Treatment of Early Incision Deep Infection after Posterior Lumbar Internal Fixation and Fusion [J]. Journal of Dali University, 2022, 7(4): 57-59.
[14]	Gao Guoxi, Lian Xingye, Liu Weibing, Zhang Jun, Hu Jianhua, Lei Yunkun, Yang Zhao, Zhang Chao. Analysis of Clinical Treatment of Lumbar Brucellosis [J]. Journal of Dali University, 2022, 7(4): 60-62.
[15]	Peng Yunchuan, Luo Ting, Qi Lin, Ma Hongbing. Clinical Application of Improved Preoperative Preparation Method for DAA Total Hip Arthroplasty [J]. Journal of Dali University, 2022, 7(4): 63-65.