Abstract: Objective: To investigate the indigenous medicinal insect "Koudai Chong" used by the Jingpo ethnic group in western
Yunnan, aiming at identifying its zoological origin, usage methods, and medicinal efficacy. The research provides reference for the
exploration and documentation of traditional medicinal animal resources. Methods: Interviews with local people, field investigation,
specimen collections, morphological classification studies, and molecular biological techniques were employed to study the origin,
ecological environment, morphological characteristics, medicinal processing and usage methods, efficacy, and molecular markers of
"Koudai Chong". Results: The origin of "Koudai Chong" was identified as Atypus sacculatus, a species of the genus Atypus belonging to
the family Atypidae in the order Araneae. Its COI sequence length was found to be 637 bp. Traditionally, it is mainly used for treating
pediatric malnutrition （ganji）. Conclusion: This research provides significant evidence for the identification of the origin and the
medicinal development of "Koudai Chong", a traditional medicinal material used by the Jingpo ethnic group in western Yunnan.

Key words: medicinal spider, Atypidae, Atypus sacculatus, DNA barcode