一种纯化茶皂素的方法

doi:10.3863/j.issn.1000-2634.2001.01.018

西南石油大学学报(自然科学版) ›› 2001, Vol. 23 ›› Issue (3): 61-62.DOI: 10.3863/j.issn.1000-2634.2001.01.018

一种纯化茶皂素的方法

赵晓东

西南石油学院化学化工学院,四川南充 637001

收稿日期:2000-12-02 修回日期:1900-01-01 出版日期:2001-08-20 发布日期:2001-08-20
通讯作者: 赵晓东

THE METHOD OF PURIFICATION FOR TEA SAPONIN

ZHAO Xiao-dong

Southwest Petroleum Inst, Nanchong Sichuan 637001, China

Received:2000-12-02 Revised:1900-01-01 Online:2001-08-20 Published:2001-08-20
Contact: ZHAO Xiao-dong

摘要/Abstract

摘要： 为了克服现有技术的缺陷,提高茶皂素的纯度和质量,扩展其应用范围,将市售的粗茶皂素配制成5%的水溶液,采用国产聚苯乙烯型树脂在pH=4进行吸附处理。0.1%氢氧化钠水溶液脱除被吸附的杂质和色素,以80%的甲醇为洗脱液,减压蒸馏回收甲醇,将残留物浓缩烘干,得到纯度95.1%、泡沫的罗氏迈尔高度达181 mm,溶血指数为1∶75000的产品。此茶皂素的纯化方法所用树脂选择性强,吸附量大,解吸和吸附容易,操作简便,成本低廉,生产周期短,有重要的实际应用价值。

关键词: 茶皂素, 纯化, 吸附树脂

Abstract: In order to overcome the present defects in technology, enhance the purity and quality of tea saponin, enlarge its application range, 5% aqueous solution of crude tea saponin is prepared and then is adsorbed by polystyrene resin under pH=4. 0.1%NaOH aqueous solution is used to remove the impurities and coloring matter which are adsorbed, and 80%(v/v) methanol solution as the eluant of the tae saponin. The produced tea saponin has the purity 95. 1%, Ross-Miles foam height 181mm, and haemolysis index 1∶75000. The resin used in this purification has strong selectivity, large adsorbance, easy of
desorption and adsorption, simple operation, low cost and short period of production. It is valuable for practical application.

Key words: tea saponin, purification, polymeric adsorbent

中图分类号: