西南石油大学学报(自然科学版) ›› 2009, Vol. 31 ›› Issue (5): 117-120.DOI: 10.3863/j.issn.1674-5086.2009.05.025

• 石油与天然气工程 • 上一篇    下一篇

细胞疏水性对采油微生物运移吸附作用的影响

刘洋1 李蔚1 单厚菲2 王蕊1   

  1. 1.中国石油大庆油田有限责任公司勘探开发研究院,黑龙江大庆163712;2.黑龙江大学生命科学院,黑龙江哈尔滨150080
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-10-20 发布日期:2009-10-20

EFFECTS OF CELL SURFACE-HYDROPHOBICITIES ON MIGRATION AND ADHENSION OF OIL RECOVERING MICROBE

LIU Yang1 LI Wei1 SHAN Hou-fei2 WANG Rui1   

  1. 1.Exploration and Development Institute,Daqing Oilfield Company Limited,CNPC,Daqing Heilongjiang 163712,China;2.Life Science College of Heilongjiang University,Harbin Heilongjiang 150080,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-10-20 Published:2009-10-20

摘要: 微生物采油的驱油机理是采油微生物在油藏中通过向原油运移并吸附于岩石表面。在运移吸附过程中,细胞的疏水性起着重要作用。从油田采出液样品中分离筛选得到5株能以原油为唯一碳源生长的菌株,分别命名为DQ112、1507、HX、QF、CU。利用微生物粘着碳氢化合物法(MATH),对采油菌种细胞表面疏水性及其环境因子进行研究,结果表明:细胞疏水性随培养时间、碳源的种类、环境温度与pH值的变化而改变,添加一定量的脂肽类生物表面活性剂可提高菌体细胞表面疏水性。

关键词: 微生物采油, 表面疏水性, 吸附

Abstract: Microorganisms in oil reservoir can enhance oil recovery by moving to crude oil and adhensing to the surface of rocks.Cell-surface hydrophobicity plays an important role in the course of movement and adhension.Five strains,named DQ11-2,1507,HX,QF,CU,which can grow with oil as the sole carbon source,are isolated and purified from the produced liquids in oilfield.The method of microorganism adhering to hydrocarbon (MATH) is used to study the bacteria Cell-surface hydrophobicity of the strains and environmental influence factors.The result shows hydrophobicity changes with the difference of cultivation time,carbon source,temperature and pH value.The cell-surface hydrophobicity of bacteria is increased by the addition of certain amount of Lipopeptide Biosurfactant.

Key words: MEOR, surface-hydrophobicities, adhension

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