西南石油大学学报(自然科学版)

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Study on the Selection and Fermentative Condition of Bacterium about
Diesel Desulfuration

Tang ke, Hong Xin   

  1. School of Chemical and Environmental Engineering,Liaoning University of Technology,Jinzhou,Liaoning 121001,China
  • Online:2013-06-01 Published:2013-06-01

Abstract:

First,28 strains were selected by using dimethyl sulfoxide as the sole sulfur source,then HT1 strain was selected
which has high desulfurization ability by using dibenzonthiophene dibenzothiophene(DBT)as the sole source of sulfur. Strain
liquid fermentation of the HT1 reveals that the optimum pH and temperature for its growth is 6.57.5 and 30 ℃,respectively.
The HT1 strain could be inhibited under acid or alkaline condition and the ability of DBT degradation decreases. The growth
of the strain prospered with glucose as carbon source,but the substrate could inhibit its growth. The HT1 strain growth and
its DBT degradation ability were slightly lower with glycerol as carbon source and the substrate cannot inhibit its growth. The
HT1 strain can better utilize organic nitrogen sources than inorganic ones;however,it has the best DBT degradation effect with
ammonium nitrate as nitrogen source. Therefore,we come to the conclusion that the best carbon source is 5 g/L glycerol and
the best nitrogen source is 2 g/L ammonium nitrate.

Key words: DBT, diesel, desulfurization, strains, fermentation