大理大学学报 ›› 2022, Vol. 7 ›› Issue (4): 8-11.

• 药学 • 上一篇    下一篇

基于GC-MS的黑水虻幼虫与成虫脂溶性成分对比研究

卿 晨,陈金虎,李伯隆,刘 衡,张成桂   

  1. (1.大理大学药学院,云南大理 671000;2.云南省昆虫生物医药研发重点实验室,云南大理 671000)

  • 收稿日期:2022-01-10 修回日期:2022-02-13 出版日期:2022-04-15 发布日期:2022-05-27
  • 通讯作者: 张成桂,教授,博士,E-mail:chenggui_zcg@163.com。
  • 作者简介:卿晨,硕士研究生,主要从事药物制剂研究。
  • 基金资助:
    云南省重大科技专项基金项目(202002AA100 007)

Comparative Analysis of Liposoluble Constituents in the Larvae and Adults of Black Soldier Fly by GC-MS

Qing Chen, Chen Jinghu, Li Bolong, Liu Heng, Zhang Chenggui   

  1. (1. College of Pharmacy, Dali University, Dali, Yunnan 671000, China;2.Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, Yunnan 671000, China)

  • Received:2022-01-10 Revised:2022-02-13 Online:2022-04-15 Published:2022-05-27

摘要:

[摘要] 目的:分析并比较黑水虻幼虫与成虫的脂溶性成分。方法:利用索氏提取法分别提取黑水虻幼虫、成虫脂溶性成分,运用气相色谱-质谱法(GC-MS)进行鉴定分析,并采用面积归一化法测定两者成分中化合物的相对含量。结果:从黑水虻幼虫脂溶性成分中检出47个峰,鉴定出22种化合物,占总离子流峰面积的47.31%;从黑水虻成虫脂溶性成分中检出21个峰,鉴定出16种化合物,占总离子流峰面积的62.47%。结论:烃类化合物和脂类化合物是黑水虻油脂的特征性成分,成虫体内含有较多的脂肪酸,而幼虫体内含有大量烃类和酚类化合物,为黑水虻的资源利用和开发提供一定的参考依据。

关键词:

"> ">[关键词] ">黑水虻;幼虫;成虫;脂溶性成分;气相色谱-">质谱法

Abstract:

〔Abstract〕 Objective: To analyze and compare the differences of liposoluble constituents of the larvae and adults of black soldier fly. Methods: The liposoluble constituents of the larvae and adults of black soldier fly were extracted by Soxhlet extraction method and determined by using GC-MS, the area normalization method was used to analyze the relative amounts of compounds between them. Results: Totally 47 peaks were detected from the larvae of black soldier fly and 22 chemical compounds of which were identified, accounting for 47.31% of the total content of liposoluble components; 21 peaks were detected from the adults of black soldier fly, and 16 chemical compounds were identified, accounting for 62.47% of the total content of liposoluble components. Conclusion: Hydrocarbons and lipid compounds are the characteristic components of the oil of black soldier fly. The adult contains more aliphatic acids, while the larvae contain a large number of olefin and phenolic compounds, which provide a certain reference for the resource utilization and development of black soldier fly.

Key words:

"> ">〔Key words">〕 black soldier fly ">; larvae">; adult">; liposoluble constituents">; GC-MS

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