〔Abstract〕Objective：To investigate the potential active components and mechanisms of total flavonoids from Sargentodoxa cuneata(TFSc) against ulcerative colitis (UC). Methods: Based on the components of TFSc identified by liquid chromatography-mass spectrom ⁃etry in a previous study, molecular docking was performed using bile acid receptors , including farnesoid X receptor (FXR), pregnane Xreceptor (PXR), Takeda G protein-coupled receptor 5 (TGR5), and vitamin D receptor (VDR), as targets to screen potential active com ⁃ponents with favorable binding affinities . Six identified representative components were selected for network pharmacology analysis , in⁃cluding target prediction, construction of protein-protein interaction (PPI) networks, Gene Ontology (GO) functional analysis, and KyotoEncyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis . Results: A total of 73 potential active components with fa⁃vorable binding affinities to the four bile acid receptors were identified . All six representative components exhibited binding affinitiesof ≤−7 .4 kcal/mol to the bile acid receptors . Network pharmacology analysis identified 38 core targets, which were mainly involved inthe PI3K-Akt, MAPK, Ras, ErbB, JAK-STAT, and HIF-1 signaling pathways, as well as biological processes including inflammatoryresponses, immune responses, oxidative stress, cell proliferation, and mucosal repair . Conclusion: TFSc may exert anti-UC effects byacting on bile acid receptors and regulating multiple targets and signaling pathways involved in bile acid metabolism , immune inflam⁃mation, and intestinal mucosal repair . These findings provide a theoretical basis for further elucidating the pharmacodynamic materialbasis and mechanism of Sargentodoxa cuneata against UC .

〔Abstract〕Pharmacokinetics is a core course in life sciences and pharmacy . In response to the issues in traditional teaching , such asinsufficient integration of ideological and political elements with professional knowledge , teaching content lagging behind industrial de⁃velopment, and students´ weak innovative thinking , the curriculum team constructed a dual-driven curriculum reform system of "cur⁃riculum ideological and political education for value shaping + case teaching for empowerment", so as to improve the contemporaneityand cutting-edge nature of the course . Centered on ideological and political guidance , the team adopted diversified teaching modessuch as case discussion, problem-based learning and flipped classroom to innovate teaching contents and methods . It promotes thetransformation and connection between theoretical knowledge , research frontiers and practical application , and realizes the high unifica⁃tion of knowledge transmission , ability cultivation, and value shaping . Meanwhile, with vivid cases and immersive teaching , the courseenhances its attractiveness and contemporary appeal , and helps cultivate compound pharmaceutical talents with lofty professional eth ⁃ics and profound professional competence .

〔Abstract〕Objective：To investigate the dynamic changes in coagulation function and their association with 30-day prognosis in pa⁃tients with severe traffic trauma receiving massive transfusion on the Yunnan-Xizang route , and to evaluate the predictive value ofearly coagulation indicators for poor prognosis . Methods: A retrospective cohort study was conducted involving 205 patients with se⁃vere traffic trauma who received massive transfusion at Unit 32268 of the Chinese People´s Liberation Army from January 2023 to De⁃cember 2024. According to their 30-day outcomes, the patients were divided into a survival group (n=144) and a death group (n=61).Prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR), fibrinogen (FIB), plateletcount (PLT), and D-dimer levels were measured within 10 min after admission (T0) and at 6 h (T1), 24 h (T2), and 72 h (T3) after mas⁃sive transfusion . Coagulation indicators and major clinical outcomes were compared between the two groups at each time point . General⁃ized estimating equations were used to analyze dynamic trends , multivariable Logistic regression was performed to identify factors asso ⁃ciated with poor prognosis, and receiver operating characteristic (ROC) curves were used to evaluate the predictive performance of co ⁃agulation indicators at T0 . Results: At T0, PT, APTT, INR, and D-dimer levels were significantly higher, whereas FIB and PLT levelswere significantly lower, in the death group than in the survival group (P<0 .001). Significant between-group differences persisted forthese indicators at T1-T3, with a significant group-by-time interaction (P<0 .001). The incidences of trauma-induced coagulopathy ,multiple organ dysfunction syndrome , and sepsis, as well as ICU length of stay, total hospital stay, and transfusion volume, were higherin the death group than in the survival group (P<0 .05). Multivariable Logistic regression analysis showed that the Injury Severity Score ,PT, INR, and D-dimer at T0 were independent risk factors for poor prognosis , whereas FIB at T0 was a protective factor (P<0 .05). ROCanalysis showed that the INR at T0 had the highest predictive value for poor 30-day prognosis, with an area under the curve (AUC) of0.85 (95% CI: 0.79-0.91). Conclusion: Patients with severe traffic trauma receiving massive transfusion on the Yunnan-Xizang routeexhibit marked coagulation dysfunction early after admission , and coagulation abnormalities persist longer in patients with poor progno ⁃sis . Elevated PT, INR, and D-dimer levels and decreased FIB levels at T0 are independently associated with poor 30-day prognosis,with INR showing good early predictive value . Dynamic monitoring of coagulation function may facilitate early risk stratification and co ⁃agulation management in patients with severe trauma at high altitude .

〔Abstract〕Objective：To analyze the current clinical application status and characteristics of acupoint application as an adjunctivetherapy for pediatric Mycoplasma pneumoniae pneumonia (MPP). Methods: A systematic search was conducted in Chinese and Englishdatabases, including CNKI, Wanfang, VIP, China BioMedical Literature Database , and Web of Science, covering the period from Janu⁃ary 1, 2015 to May 31, 2025. Association rules, complex networks and system clustering analyses were conducted using SPSS Modeler18.0 and SPSS 26.0. Results: A total of 90 articles were included, from which 112 prescriptions covering 10 syndrome types were ex⁃tracted . The high-frequency acupoints reported were Feishu (BL13, 31.56%), Tanzhong (CV17, 17.49%), and Tiantu (CV22, 14.07%).The high-frequency herbs were Ephedrae Herba (Mahuang, 7.79%), Armeniacae Semen Amarum (Kuxingren, 6.12%), Sinapis AlbaeSemen (Baijiezi, 5.42%), Gypsum Fibrosum (Shigao, 4.59%), and Descurainiae Semen (Tinglizi, 4.17%).Medicinal properties weredominated by warm and cold natures, while medicinal flavors were mainly pungent , sweet and bitter . The high-frequency acupointswere clustered into five groups, and high-frequency herbs into four groups . Association rule analysis revealed 14 strongly associatedacupoint combinations, with the core combination of Feishu-Tanzhong-Tiantu , and 25 strongly associated herb combinations , with thecore formula consisting of Mahuang-Baijiezi-Tinglizi-Shigao-Kuxingren-Dahuang-Huangqin-Yuxingcao-Jinyinhua . The most fre⁃quently reported dosage form was paste , with vinegar or ginger juice as the main excipients . Conclusion: According to the literature,acupoint application as an adjunctive therapy for pediatric MPP predominantly selects the Back-Shu point (Feishu) in combinationwith the Conception Vessel acupoints (Tanzhong and Tiantu). The medicinal compatibility presents the characteristics of "pungent me ⁃dicinals for dispersing and diffusing , bitter medicinals for descending and purging , sweet medicinals for moderating and alleviating".The prescription design, in terms of acupoint selection , medication, dosage forms and excipients, is closely associated with syndromedifferentiation and treatment .

〔Abstract〕Professional master´s education in clinical medicine is an important pathway for cultivating high-level applicationoriented medical professionals, and collaboration between medical institutions and educational institutions is essential for improvingthe quality of talent cultivation . In response to practical challenges in stakeholder collaboration , interaction and coordination, resourcesharing, and shared value objectives between universities and hospitals , a collaborative medical education and training mechanism forprofessional master´s students in clinical medicine was constructed from the perspective of value co-creation . Based on a fourdimensional framework comprising multiple stakeholders , interactive platforms, shared resources, and common goals, a core trainingcycle consisting of motivation , resource sharing, process collaboration, and evaluation and feedback was established . Practical pathwayswere proposed across three stages: pilot implementation, institutional integration, and cultural cultivation . Through stakeholder engage⁃ment, integration of university and hospital resources , whole-process collaboration , and dynamic evaluation and feedback , universitiesand hospitals can shift from formal cooperation toward deep collaboration , thereby achieving synergistic improvement in talent cultiva ⁃tion and medical service capacity . The perspective of value co-creation provides new theoretical insights and practical pathways foraddressing structural challenges in collaborative medical education for professional master´s students in clinical medicine and offers areference for constructing a high-quality collaborative medical talent training system .

〔Abstract〕With the development of the New Medical Science initiative and advances in clinical laboratory technology , the traditionalteaching model of Clinical Parasitology and Parasitological Laboratory Techniques has certain limitations in the integration of theoreti ⁃cal knowledge with clinical practice , practical teaching settings, and assessment and evaluation . Based on the concept of outcomebased education (OBE), a systematic curriculum reform was implemented for medical laboratory science students at a local university ,focusing on curriculum objectives, teaching content, teaching mode, and the assessment and evaluation system . A teaching frameworkof "theoretical foundation-practical training-clinical application-innovation and development" was established , incorporating blendedonline and offline teaching, case discussions, virtual simulation-based practice , and diversified assessment throughout the teaching pro⁃cess . Fifty students from the 2022 cohort were selected as the control group , and 49 students from the 2023 cohort were selected as thereform group . The effects of the reform were evaluated in terms of curriculum satisfaction , final examination scores, and innovation andpractical achievements . The results showed that curriculum satisfaction and final examination scores increased in the reform group . Themean score increased from 73.9 to 76.7, the minimum score increased from 48 to 62, and the number of students who failed decreasedto zero . Innovation and entrepreneurship projects and achievements in disciplinary competitions also showed positive changes , includ⁃ing a breakthrough in obtaining a national-level competition award . Curriculum reform based on the OBE concept can help enhancestudents´ learning initiative, strengthen the integration of theoretical knowledge with clinical practice , and promote the development ofpractical abilities and innovative literacy , providing a reference for teaching reform of related courses in medical laboratory science pro ⁃grams at local medical colleges and universities .

〔Abstract〕Objective : To analyze the epidemiological and molecular biological characteristics of the cluster epidemic of norovirus in ⁃fection caused by the G Ⅱ.13［P21］variant in Yuxi City in 2025, supplement the epidemic data of rare norovirus genotypes in this re ⁃gion, and improve the prevention and control strategies for norovirus in schools . Methods: Through questionnaires and on-site verifica⁃tion, information on the onset of cases and epidemic data were collected . Current cases and environmental specimens were collected forpathogen detection, and positive specimens were subjected to gene sequencing and homology analysis . Results: From April 6 to 10,2025, a total of 11 norovirus infection cases were reported in a school in Yimen County , all among enrolled students, with an attack rateof 13.75% (11/80). The highest attack rate was observed in the sixth grade , where the index case identified (88 .89%, 8/9), and in MaleDormitory 1 for boys where the index case resided had the highest attack rate (75 .00%, 6/8). Risk factor analysis of food exposures foreach meal from April 6 to 8 showed no statistically significant differences between the case group and control group for any food item（P＞0.05）. Gene sequencing identified the strain as norovirus variant G Ⅱ.13［P21］. Conclusion: This is the first cluster outbreakcaused by the norovirus GⅡ.13［P21］variant among students in Yuxi City . The possibility of transmission through contact and aerosolmixing is relatively high . Active monitoring and health education on norovirus in schools should be strengthened .

〔Abstract〕Objective : To systematically review risk prediction models for gestational diabetes mellitus (GDM) developed in Chinesepopulation . Methods: PubMed, Web of Science, Embase, Cochrane Library, CBM, Wanfang, CNKI, and VIP databases were searchedfor studies published from January 2010 to December 2024. Two researchers independently screened the literature , extracted data, andassessed the risk of bias, followed by qualitative analysis . Results: A total of 48 studies were included, comprising 42 model develop⁃ment studies without external validation , 5 development studies with external validation , and 1 external validation study . 47 studieswere rated as having a high risk of bias , and one study had an unclear risk . All model development studies evaluated model discrimina ⁃tion using the area under the curve (AUC), which ranged from 0.659 to 0.988. 38 model development studies reported calibration perfor⁃mance, which was generally acceptable . 12 studies constructed clinical decision curves , indicating potential net benefit . Internal valida⁃tion was performed in 27 studies, while only 5 studies conducted external validation . Conclusion: Although most prediction modelspossessed certain discriminatory capacity , 97.9% (47/48) of the included studies were assessed as having a high risk of bias . Addition⁃ally, internal validation was insufficient and external validation was largely absent , indicating that the robustness of the existing evi⁃dence is markedly inadequate . The findings of this study are only exploratory in nature , and the generalizability and applicability ofthese models require further validation under rigorous methodological standards .

〔Abstract〕Objective : To explore the application effect of the conceive-design-implement-operate (CDIO) teaching mode in thetraining of midline catheter (MC) placement and maintenance . Methods: A total of 80 trainees who participated in MC placement andmaintenance training course at the 920^th Hospital of the Joint Support Force of the People´s Liberation Army of China from 2023 to2024 were enrolled and divided into the control group and the experimental group using a historical control design , with 40 trainees ineach group . The control group adopted the conventional teaching , while the experimental group adopted the CDIO teaching . The teach⁃ing effect was evaluated using the General Information Questionnaire , the Nursing Staff Autonomous Learning Ability Rating Scale , theTeaching Satisfaction Questionnaire , and the Patient Catheter Usage Record . Results: After the training, the total score of autonomouslearning ability and the scores of each dimension , the scores of each dimension of teaching satisfaction and the theoretical and opera ⁃tional scores of the trainees in the experimental group were all higher than those in the control group (P<0 .05). The one-time catheter⁃ization success rate of the experimental group (85 .94%) was higher than that of the control group (64 .41%). There were statistically sig⁃nificant differences in the number of punctures , puncture methods, puncture sites, catheterization duration, indwelling duration, extuba⁃tion types, and the occurrence of complications between the two groups (P<0 .05). Conclusion: The CDIO teaching mode is superior toconventional teaching in the training of MC placement and maintenance . It can effectively improve trainees´ theoretical and practicalabilities, enhance their autonomous learning ability and teaching satisfaction , and thus is worthy of wide promotion in clinical nursingteaching . Catheter-related outcomes require further validation .