大理大学学报 ›› 2022, Vol. 7 ›› Issue (4): 34-39.

• 基础医学 • 上一篇    下一篇

7-羟基异黄酮对HaCaT细胞增殖活性和UVB诱导光损伤的影响

段 奕,倪昌雯,王敏华,孙素姣,黄 玲   

  1. (1.大理大学临床医学院,云南大理 671000;2.大理大学第一附属医院,云南大理 671000)

  • 收稿日期:2021-12-29 修回日期:2022-01-16 出版日期:2022-04-15 发布日期:2022-05-27
  • 通讯作者: 黄玲,教授,E-mail:huanglingx@126.com。
  • 作者简介:段奕,硕士研究生,主要从事光老化、皮肤病理相关研究。
  • 基金资助:
    云南省教育厅科学研究基金项目(2018JS411;2021Y426);云南省卫生和计划生育委员会医学学科学术带头人培养计划(D-2017009)

Effects of the 7-Hydroxyisoflavone in the Proliferative Activity of HaCaT Cells and UVB-induced Photodamage

Duan Yi, Ni Changwen, Wang Minhua, Sun Sujiao, Huang Ling   

  1. (1.Clinical College, Dali University, Dali, Yunnan 671000,China; 2. The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China)

  • Received:2021-12-29 Revised:2022-01-16 Online:2022-04-15 Published:2022-05-27

摘要:

[摘要] 目的:探讨7-羟基异黄酮对HaCaT细胞的增殖活性和中波紫外线(UVB)诱导光损伤的影响及作用机制。方法:通过细胞计数法检测不同浓度7-羟基异黄酮对HaCaT细胞增殖活性的影响;用200 μmol/L 7-羟基异黄酮预处理HaCaT细胞后给予30 mJ/cm2 的UVB照射,荧光法检测活性氧(ROS)生成量,用蛋白质印迹法检测细胞凋亡相关蛋白聚ADP-核糖聚合酶1(PARP1)和半胱氨酸蛋白酶-8(Caspase-8)的表达。结果:200 μmol/L 的7-羟基异黄酮对HaCaT细胞增殖活性作用最明显,可降低UVB诱导的ROS生成量,抑制PARP1和Caspase-8的表达。结论:7-羟基异黄酮能减轻UVB所致的光损伤,对细胞具有光保护作用,可预防UVB造成的皮肤损伤。

关键词:

"> ">[关键词] 7-">羟基异黄酮, 中波紫外线, HaCaT">细胞, 活性氧, 细胞凋亡

Abstract:

〔Abstract〕 Objective: To investigate the effects of the 7-Hydroxyisoflavone on the proliferative activity of HaCaT cells and the UVB-induced photodamage, as well as its mechanism. Methods: The cell counting assay was used to detect the effects of different concentrations of 7-Hydroxyisoflavone on the proliferative activity of HaCaT cells. Next, the HaCat cells were pretreated with 200 μmol/L 7-Hydroxyisoflavone and were exposed to UVB(30 mJ/cm2). Then, the production of reactive oxygen species(ROS) was detected by fluorescence method. The expressions of PARP1 and Caspase-8 were detected by Western blot. Results:The 7-Hydroxyisoflavone had the most obvious effect on the proliferative activity of HaCaT cells at the concentration of 200 μmol/L, and it could reduce the production of UVB-induced ROS. Besides, it could also inhibit the expressions of the PARP1 and Caspase-8.Conclusion:The 7-Hydroxyisoflavone can reduce photodamage caused by UVB and has a photoprotective effect on cells, which can be used to prevent skin damage caused by UVB.

Key words:

"> ">〔Key words">〕 7-Hydroxyisoflavone">, UVB">, HaCaT cells">, reactive oxygen species">, apoptosis

中图分类号: