关键词: font-family:宋体, ">美洲大蠊提取物, font-family:", ">Cfont-family:宋体, ">Ⅱfont-family:", ">-3font-family:宋体, ">, font-family:", ">K562font-family:宋体, ">细胞, 增殖, 细胞周期, font-family:", ">p53font-family:宋体, ">蛋白

Abstract: Objective： To investigate the effect of Periplaneta americana extract CⅡ-3 on the proliferation of human leukemia cell K562. Methods： K562 cells were treated with different concentrations of Periplaneta americana extract CⅡ-3， and the effects of CⅡ-3 on the proliferation of K562 cells were detected by CCK-8 assay. Colony formation assay was used to detect its effect on proliferation of K562 cells. The cell cycle of K562 cells was detected by flow cytometry. The expression of phosphorylated p53 protein was detected by Western blot. Results：Periplaneta americana extract CⅡ-3 could inhibit the proliferation of K562 cells， and the optimal concentration and duration of action were 80 μg/mL and 72 h respectively. Periplaneta americana extract CⅡ-3 reduced the colony formation rate of K562 cells. The proportion of K562 cells blocked in G0/G1 phase increased， while that in S phase decreased. The expression of phosphorylated p53 protein was up-regulated. Conclusion： Periplaneta americana extract CⅡ-3 inhibits the proliferation of K562 cells by regulating p53 protein.

Key words: font-family:Times New Roman, ">Periplaneta americana extract Cfont-family:Times New Roman, ">Ⅱfont-family:Times New Roman, ">-3font-family:Times New Roman, ">, font-family:Times New Roman, "> K562 cellsfont-family:Times New Roman, ">, font-family:Times New Roman, "> proliferationfont-family:Times New Roman, ">, font-family:Times New Roman, "> cell cyclefont-family:Times New Roman, ">, font-family:Times New Roman, "> p53 protein