关键词:

"> font-size:10.5pt, ">溃疡性结肠炎, 大乌泡, 闭合蛋白, 黏蛋白2, 紧密连接蛋白-1

Abstract:

Objective： To investigate the therapeutic effect and mechanism of Miao medicine Rubus pluribracteatus on dextran sodium sulfate （DSS）-induced ulcerative colitis （UC） in mice. Methods： Fifty male C57BL/6 mice were randomly divided into control group， model group， mesalazine group， R. pluribracteatus low-dose group and R. pluribracteatus high-dose group， with 10 mice in each group. The control group drank water for 7 days， and the other groups were given 3%DSS solution for 7 days to construct UC mouse model. Thereafter， each group was gavaged for 7 days. During the modeling and administration period， the disease activity index（DAI） of mice in each group was measured. After the last administration， blood extraction of inner canthus was completed and the mice in each group were sacrificed and the whole colon（from cecum to anus） was cut to measure the length of the colon. Hematoxylin and eosin staining was used to observe the pathological morphology， enzyme linked immunosorbent assay was used to detect the levels of tumor necrosis factor-α（TNF-α） and diamine oxidase（DAO） in serum， and Western blot was used to detect the expression of Occludin， mucin 2（Muc2）， and zonula occludens-1（ZO-1） in colon tissue. Results： R. pluribracteatus could reduce DAI scores of UC mice， increase the length of the colon，alleviate the histological changes of colon tissue， decrease the levels of TNF-α and DAO in serum， and upregulate the expression levels of Occludin， Muc2  and ZO-1. Conclusion： Miao medicine R. pluribracteatus may treat UC by promoting the expression of Occludin， Muc2 and ZO-1 proteins and improving intestinal mucosal barrier function.

Key words:

"> font-size:10.5pt, ">ulcerative colitis, Rubus pluribracteatus, Occludin, mucin 2 , zonula occludens-1font-size:10.5pt, ">