大理大学学报 ›› 2023, Vol. 8 ›› Issue (8): 54-58.

• 基础医学 • 上一篇    下一篇

高盐干预对人主动脉血管平滑肌细胞增殖和迁移的影响

邓文斌,黄仕琼,王 雪,李利华*   

  1. (大理大学第一附属医院老年病科,云南大理 671000)

  • 收稿日期:2022-11-11 修回日期:2023-01-20 出版日期:2023-08-15 发布日期:2023-07-11
  • 通讯作者: 李利华,教授,博士,E-mail:lilihuayncn@163.com。
  • 作者简介:邓文斌,硕士研究生,主要从事动脉硬化研究。
  • 基金资助:
    国家自然科学基金项目(81860084;82260076)

Effects of High Salt Intervention on Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cell

Deng Wenbin,Huang Shiqiong, Wang Xue,Li Lihua*   

  1. (Department of Geriatrics, The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China)

  • Received:2022-11-11 Revised:2023-01-20 Online:2023-08-15 Published:2023-07-11

摘要: 目的:研究不同浓度Na+干预对人主动脉血管平滑肌细胞(HAVSMC)增殖和迁移的影响。方法:原代培养HAVSMC,传代至第5代后用不同浓度的Na+(132、137、142、147、152、157、162、167 mmol/L)干预,培养24、48、72、96 h后进行细胞增殖和迁移实验。CCK-8法检测HAVSMC的增殖活性;实时荧光定量聚合酶链反应检测增殖细胞核抗原(PCNA)表达,分析HAVSMC的增殖能力;划痕实验评估HAVSMC的迁移能力。结果:与132 mmol/L Na+对照组相比,培养72 h后,137~167 mmol/L Na+干预均可促进HAVSMC的增殖能力(P<0.05),Na+浓度为152 mmol/L时具有显著的促增殖作用(P<0.05),且可促进 HAVSMC的迁移。结论:高盐干预可促进HAVSMC增殖和迁移。

关键词: font-family:宋体, ">盐;人主动脉血管平滑肌细胞;增殖;迁移

Abstract: Objective: To investigate the effects of different concentrations of Na+ intervention on the proliferation and migration of human aortic vascular smooth muscle cell(HAVSMC). Methods: HAVSMC were cultured as primary cultures and were passaged to the fifth generation. Cells were intervened with different concentrations of Na+(132, 137, 142, 147, 152, 157, 162, and 167 mmol/L), and cell proliferation and migration experiments were conducted at 24, 48, 72, and 96 hours after the intervention. Proliferation activity of HAVSMC was detected by the CCK-8 method, and proliferating cell nuclear antigen(PCNA) expression was analyzed by real-time fluorescence quantitative polymerase chain reaction in order to evaluate proliferation ability of HAVSMC. Additionally, scratch assay was employed to evaluate the migration ability of HAVSMC. Results: Compared with the 132 mmol/L Na+ control group, after 72 hours of cultivation, 137-167 mmol/L Na+ intervention promoted the proliferation ability of HAVSMC. Among all intervention groups(P<0.05), Na+ concentration of 152 mmol/L had a significant pro-proliferation effect(P<0.05) and promoted the migration of HAVSMC. Conclusion: High salt intervention can promote the proliferation and migration of HAVSMC.

Key words: font-family:Times New Roman, ">saltfont-family:Times New Roman, ">;font-family:Times New Roman, "> human aortic vascular smooth muscle cellfont-family:Times New Roman, ">;font-family:Times New Roman, "> proliferationfont-family:Times New Roman, ">;font-family:Times New Roman, "> migration

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