Abstract:

Objective：To observe the content of iridoid glycoside in Gentiana crassicauls growing at different altitudes and to provide
a scientific basis for the cultivation of this herb and its suitable planting areas. Methods：A HPLC method was used to measure the
content of four active components in Gentiana crassicauls at different altitudes, and chemometric methods were applied to analyze
HPLC fingerprinting and chemical recognition. Results：The content of the active components in this medical plant from 26 product
batches was in compliance with relevant standards of quality. The similarity of chromatographic fingerprints was greater than 0.9. The
experimental results indicated that the influence of altitude variations on the content of iridoid glycoside was not significant.
Conclusion：The iridoid glycoside content in the Gentiana crassicauls roots at the altitude of 2 700 m is higher than that at other
altitudes which is suitable for the cultivation of Gentiana crassicauls ．

Key words: Gentiana crassicauls, altitudes, iridoid glycoside