Study on Anti-Oxidative Stress Effect of
Xigui Crude Polysaccharide for Hypoxic Mice

Abstract

Abstract:

Objective： To study anti-oxidative stress effect of Xigui crude polysaccharide for hypoxic mice. Methods： Kunming mice were randomly divided into normal group， hypoxic model group， salidroside group（360 mg/kg）， Xigui crude polysaccharide low dose group（100 mg/kg）， medium dose group （200 mg/kg） and high dose group （400 mg/kg）. The survival time of the first batch of mice put in hypoxic environment was measured. The superoxide dismutase（SOD） activity， glutathione peroxidase Px（GSH-Px） activity， malondialdehyde （MDA） content， acetylcholinesterase （AChE） activities in liver tissue and serum， and serum nitric oxide （NO） content in the second batch of mice were measured. Results： Each dose group of Xiguei crude polysaccharide could prolong the survival time of mice, significantly decrease the content of MDA in serum（P<0.01）， and up-regulate the activities of SOD and GSH-Px in serum， and the high dose group had the best effect（P<0.05）. The medium and high dose groups of Xiguo crude polysaccharide could significantly decrease the content of NO and the activity of AChE in serum of mice（P<0.05）， significantly increase the activity of SOD in liver tissue of mice（P<0.05）， decrease the content of MDA in liver tissue of mice and increase the activity of GSH-Px in liver tissue of mice， and the high dose group had the best effect （P<0.05）. The AChE activity in liver tissue of mice could be decreased in the middle dose group of Xiguei crude polysaccharide（P<0.05）. Conclusion： Xigui crude polysaccharide can effectively prolong the survival time of mice under hypoxia and has a better anti-oxidative stress effect.

Key words: font-family:Times New Roman, ">Xigui crude polysaccharidefont-family:Times New Roman, ">；font-family:Times New Roman, "> anti-oxidative stressfont-family:Times New Roman, ">；font-family:Times New Roman, "> hypoxia">

CLC Number:

R965.2

References

〔1〕宝丽.欧洲越橘花色苷提取物抗氧化应激作用及其机制学研究〔D〕.沈阳：沈阳药科大学，2008.

〔2〕董寿堂，张旭强，胡燕，等.西归的化学成分、质量控制及药理学研究概况〔J〕.中国民族民间医药，2018，27（13）：40-42.

〔3〕田佳丽，郭美仙，刘光明，等.西归含药血清对脂多糖诱导的小鼠巨噬细胞RAW264.7炎症因子的影响研究〔J〕.药学研究，2018，37（9）：497-499.

〔4〕段金成，罗顺迪，曹祖高，等.西归粗多糖对游泳力竭小鼠的抗运动性疲劳作用〔J〕.中成药，2018，40（3）： 681-684.

〔5〕周启微，郭美仙，杨佳晶，等.西归对缩宫素所致小鼠原发性痛经的机制研究〔J〕.大理大学学报，2017，2（4）：24-27.

〔6〕董寿堂，王成军，王雨辰，等.西归抗疲劳活性组分的筛选研究〔J〕.中国药房，2015，26（1）：43-45.

〔7〕邱艳，陈莲，刘建霞，等.西归挥发油抗痛经作用研究〔J〕.井冈山大学学报（自然科学版），2019，40（4）：96-102.

〔8〕许嘉强，孙进，李艳，等.西归多糖的提取及含量测定研究〔J〕.大理学院学报，2009，8（10）：15-17.

〔9〕周庆峰，姜书纳，马亢，等.铁棍山药多糖抗疲劳及耐缺氧作用研究〔J〕.时珍国医国药，2014，25（2）：284-285.

〔10〕王赟，丁晨晨，李伟鑫，等.研究枸杞子对小鼠抗缺氧抗疲劳能力的影响〔J〕.中西医结合心血管病电子杂志，2017，5（34）：167-168.

〔11〕楚秉泉.西藏芜菁的抗缺氧功能成分分离及其作用机制研究〔D〕.杭州：浙江大学，2017.

〔12〕王燕.不同海拔两种沙蜥冬眠前后氧化应激水平的研究〔D〕.兰州：兰州大学，2017.

〔13〕张佳婵，程琳，化璟琳，等.沙棘粕醇提取物对小鼠氧化应激损伤的保护作用及机制〔J〕.中国食品学报，2019，19（7）：11-19.

〔14〕谭秀娟，李古兵，张淼，等.芫根总多糖对模拟高原缺氧小鼠的作用研究〔J〕.华西药学杂志，2020，35（2）：171-175.

〔15〕张石在.天麻提取物提高大鼠的抗缺氧能力〔J〕.现代食品科技，2019，35（7）： 76-81.

〔16〕胡尧.红景天对缺氧脑损伤的保护作用及其神经元线粒体MPTP机制的研究〔D〕.成都：成都中医药大学，2017.

〔17〕李方敏，姚灿灿，丁存宝.三唑磷对斑马鱼肝脏氧化损伤作用研究〔J〕.生物化工，2020，6（1）： 97-99.

〔18〕仲崇虎，王梦杰，魏星，等.磺胺二甲嘧啶对脊尾白虾抗氧化酶活性的影响〔J〕.江苏农业科学，2019，47（15）：205-207.

〔19〕 BAKIR T， SAYINER H S， KANDEMIRLI F. Experimental and Theoretical Investigation of Antioxidant Activity and Capacity of Thiosemicarbazones Based on Isatin Derivatives〔J〕.Phosphorus Sulfur，2018，193（8）：493-499.

〔20〕程文栋，顾玉海，赵振.不同海拔及时间下大鼠肺组织白介素及SOD、MDA的变化研究〔J〕.高原医学杂志，2018，28（4）：22-27.

〔21〕柯维旺，朱梅菊，朱洪竹，等.热敏灸对运动大鼠运动能力和心肌、骨骼肌细胞线粒体过氧化损伤的影响〔J〕.井冈山大学学报（自然科学版），2016，37（2）：83-87.

〔22〕孟祥云，汪永锋，杨丽霞，等.中药多糖抗氧化作用及其机制研究进展〔J〕.中华中医药杂志，2018，33（8）：3504-3509.

〔23〕 HE S， REHMAN H， WRIGHT G L， et al. Inhibition of Inducible Nitric Oxide Synthase Prevents Mitochondrial Damage and Improves Survival of Steatotic Partial Liver Grafts〔J〕.Transplantation，2010，89（3）：291-298.

〔24〕刁波，文晔，杨李，等.刺五加多糖对氧化应激损伤的海马神经元iNOS mRNA表达的影响〔J〕.华南国防医学杂志，2008，4（4）：15-17.

〔25〕佟双喜，郭蔚莹.当归多糖对糖尿病大鼠肾组织抗氧化能力及NOS、NO水平的影响〔J〕.中国老年学杂志，2015，

35（24）：7001-7002.

〔26〕 HAN B， GAO Y， WANG Y， et al. Protective Effect of a Polysaccharide from Rhizoma Atractylodis Macrocephalae on Acute Liver Injury in Mice〔J〕.Int J Biol Macromol，2016，87：85-91.

〔27〕刘兴琴，丁济超，李继兴，等.西归粗多糖对运动疲劳小鼠的免疫调节作用〔J〕.食品工业科技，2021，42（5）：317-321.

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Study on Anti-Oxidative Stress Effect of Xigui Crude Polysaccharide for Hypoxic Mice

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