Journal of Dali University ›› 2022, Vol. 7 ›› Issue (2): 22-28.

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Chloroplast Genome Characteristics of a Tibetan Endangered Medicinal Plant Lamiophlomis rotata

Pu Fen1, Zhang Dequan1,2*   

  1. (1.College of Pharmacy, Dali University, Dali, Yunnan 671000, China; 2. Yunnan Key Laboratory of Screening and Research

    on Anti-Pathogenic Plant Resources from Western Yunnan(Cultivation), Dali, Yunnan 671000, China)

  • Received:2021-06-25 Revised:2021-09-15 Online:2022-02-15 Published:2022-03-28

Abstract:

Objective: To reveal the complete chloroplast genome characteristics of a medicinal plant in Tibetan region, namely Lamiophlomis rotate, and explore its phylogenetic position. Methods: In this study, the Next-Generation Sequencing technology was adopted to perform DNA sequencing for this species, and its complete chloroplast genome was obtained after assembly and annotation. Meanwhile, the genome characteristics were revealed, and its phylogeny was systematically analyzed, combined with the published chloroplast genomes of related species in Lamiaceae. Results: The complete chloroplast genome of L. rotata was a typical quadripartite structure, the total length of which was totally 151 808 bp, and 107 genes were annotated. The topological structures between the Bayesian(BI) and the maximum likelihood(ML) were extremely similar, proving that Lamiophlomis and Stachys were gathered into one clade. Conclusion: The complete chloroplast genome characteristics of L. rotata were revealed for the first time, and its phylogenetic study supported the traditional treatment of regarding Lamiophlomis and Stachys as closely related genera. This study could provide scientific basis for studies such as species identification, resource conservation, and phyletic evolution of L. rotata and related species.

Key words: font-family:SimSun, ">Lamiophlomis rotatafont-family:SimSun, ">;font-family:SimSun, "> chloroplast genomefont-family:SimSun, ">;font-family:SimSun, "> phylogenyfont-family:SimSun, ">;font-family:SimSun, "> Next-Generation Sequencingfont-family:SimSun, ">;font-family:SimSun, "> medicinal plant

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