Journal of Dali University ›› 2023, Vol. 8 ›› Issue (2): 1-7.

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The Protective Effect and Mechanism of Panax notoginseng Saponins on ox-LDL-Induced Platelet Apoptosis

Ma Yongjie1, Li Weiqi1, Wu Chunting1, Huang Xinhui1, Zhou Xinyu1, Ya Fuli1, 2*   

  1. (1.College of Public Health, Dali University, Dali, Yunnan 671000, China; 2. Institute of Translational Medicine for Metabolic Diseases, Dali University, Dali, Yunnan 671000, China)

  • Received:2022-10-13 Revised:2022-11-16 Online:2023-02-15 Published:2023-03-02

Abstract: Objective: To explore the effect of Panax notoginseng saponins(PNS) on oxidized low-density lipoprotein(ox-LDL)-induced platelet apoptosis and its potential molecular mechanism in vitro. Methods: Human gel-filtered platelets were pre-treated with different concentrations of PNS (0,1,10,100 μg/mL) for 40 minutes, and then stimulated with 50 μg/mL ox-LDL for 60 minutes. Subsequently, indicators of platelet apoptosis including platelet mitochondrial membrane potential(Δψm) levels and phosphatidylserine(PS) exposure levels were detected by flow cytometry. Reactive oxygen species(ROS) levels and superoxide levels were measured by commercial ROS assay kit. The phosphorylation levels of Src and Syk proteins as well as the activation levels of Caspase-3 and Caspase-9 were detected by Western blotting. Results: Compared with the control group, ox-LDL-induced depolarization of platelets Δψm was significantly reduced in each dose group of PNS(P<0.01), ox-LDL-induced exposure level of platelet surface PS(P<0.05), total ROS levels and superoxide levels(P<0.05), Caspase-3 and Caspase-9 activation levels(P<0.05),and phosphorylation levels of Src and Syk proteins(P<0.05) were inhibited in the medium and highdose PNS groups. Conclusion:PNS may play the role of anti ox-LDL-induced platelet apoptosis by inhibiting Src/Syk/ROS signalling pathway.

Key words: font-family:Times New Roman, ">Panax notoginseng saponinsfont-family:Times New Roman, ">;font-family:Times New Roman, "> oxidized low-density lipoproteinfont-family:Times New Roman, ">;font-family:Times New Roman, "> plateletfont-family:Times New Roman, ">;font-family:Times New Roman, "> apoptosisfont-family:Times New Roman, ">;font-family:Times New Roman, "> reactive oxygen species

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