Expression of RIP140 and LCOR in High-Grade
Cervical Intraepithelial Neoplasia

Abstract

Abstract: Objective： To analyze the expression levels of RIP140 and LCOR in cytoplasm and nucleus in high-grade squamous intraepithelial lesion（HSIL） in order to find new biomarkers for the identification of cervical intraepithelial neoplasia（CIN） Ⅱ and CIN Ⅲ. Methods： Immunohistochemistry was used to detect the expression of RIP140 and LCOR in cytoplasm and nucleus in 45 cases of CIN Ⅱ， 32 cases of CIN Ⅲ， 51 cases of CIN Ⅱ and CIN Ⅲ combined morphology. Results： The expressions of RIP140 in cytoplasm and nucleus of CIN Ⅲ were higher than those of CIN Ⅱ， with statistical significance（P<0.05）. There was no statistically significant difference in the expression of LCOR in cytoplasm between CIN Ⅱ and CIN Ⅲ（P>0.05）， but the expression of LCOR in nucleus of CIN Ⅱ was higher than that of CIN Ⅲ， with statistical significance（P<0.05）. RIP140 was highly expressed in the cytoplasm of the complex morphologic samples， and the difference was statistically significant compared with CIN Ⅱ（χ2=4.868， P=0.027）. Conclusion： The high expression of RIP140 in the nucleus has an auxiliary effect in distinguishing CIN Ⅱ from CIN Ⅲ， and its high expression in the cytoplasm suggests that CIN Ⅱ may have potential coexistence with high-grade squamous intraepithelial neoplasia.

Key words: font-family:Times New Roman, ">CIN font-family:Times New Roman, ">Ⅱ；font-family:Times New Roman, "> CIN font-family:Times New Roman, ">Ⅲ；font-family:Times New Roman, "> RIP140font-family:Times New Roman, ">；font-family:Times New Roman, "> LCOR

CLC Number:

Zhi Hongfang, Chen Xin, Zhang Jianyan, Wang Chengcheng, Zhang Yun, Yang Xuantao.

Expression of RIP140 and LCOR in High-Grade Cervical Intraepithelial Neoplasia [J]. Journal of Dali University, 2023, 8(8): 59-63.

References

〔1〕 FERLAY J， SOERJOMATARAM I， DIKSHIT R， et al. Cancer Incidence and Mortality Worldwide： Sources， Methods and Major Patterns in GLOBOCAN 2012〔J〕. Int J Cancer，2015，136（5）：E359-E386.

〔2〕 FERLAY J， COLOMBET M， SOERJOMATARAM I， et al. Estimating the Global Cancer Incidence and Mortality in 2018： GLOBOCAN Sources and Methods〔J〕. Int J Cancer，2019，144（8）：1941-1953.

〔3〕王宇，宋淑芳，刘凤.我国宫颈癌流行病学特征和发病高危因素的研究进展〔J〕.中国妇幼保健，2019，34（5）：1206-1208.

〔4〕 WHITE R， MORGANSTEIN D， CHRISTIAN M， et al. Role of RIP140 in Metabolic Tissues： Connections to Disease〔J〕. FEBS Lett，2008，582（1）：39-45.

〔5〕 POWELKA A M， SETH A， VIRBASIUS J V， et al. Suppression of Oxidative Metabolism and Mitochondrial Biogenesis by the Transcriptional Corepressor RIP140 in Mouse Adipocytes〔J〕. J Clin Invest，2006，116（1）：125-136.

〔6〕 DOCQUIER A， HARMAND P O， FRITSCH S， et al. The Transcriptional Coregulator RIP140 Represses E2F1 Activity and Discriminates Breast Cancer Subtypes〔J〕. Clin Cancer Res，2010，16（11）：2959-2970.

〔7〕 LAPIERRE M， BONNET S， BASCOUL-MOLLEVI C， et al. RIP140 Increases APC Expression and Controls Intestinal Homeostasis and Tumorigenesis〔J〕. J Clin Invest，2014，124（5）：1899-1913.

〔8〕 SIXOU S， M?譈LLER K， JALAGUIER S， et al. Importance of RIP140 and LCoR Sub-Cellular Localization for Their Association with Breast Cancer Aggressiveness and Patient Survival〔J〕. Transl Oncol，2018，11（5）：1090-1096.

〔9〕 VATTAI A， CAVAILLES V， SIXOU S， et al. Investiga-tion of RIP140 and LCoR as Independent Markers for Poor Prognosis in Cervical Cancer〔J〕. Oncotarget，2017，8（62）：105356-105371.

〔10〕 LAPIERRE M， DOCQUIER A， CASTET-NICOLAS A， et al. The Emerging Role of the Transcriptional Coregulator RIP140 in Solid Tumors〔J〕. Biochim Biophys Acta，2015，1856（1）：144-150.

〔11〕 RAMACHANDRAN B. Functional Association of Oestro-gen Receptors with HPV Infection in Cervical Carcino-genesis〔J〕. Endocr Relat Cancer，2017，24（4）：R99-R108.

〔12〕 CHEN H Z， TSAI S Y， LEONE G. Emerging Roles of E2Fs in Cancer： An Exit from Cell Cycle Control〔J〕. Nat Rev Cancer，2009，9（11）：785-797.

〔13〕 SRIVASTAVA P， MANGAL M， AGARWAL S M. Understanding the Transcriptional Regulation of Cervix Cancer Using Microarray Gene Expression Data and Promoter Sequence Analysis of a Curated Gene Set〔J〕. Gene，2014，535（2）：233-238.

〔14〕 M?譈LLER K， SIXOU S， KUHN C， et al. Prognostic Relevance of RIP140 and ERβ Expression in Unifocal versus Multifocal Breast Cancers： A Preliminary Re-port〔J〕. Int J Mol Sci， 2019， 20（2）： 418.

〔15〕 ASIM M， HAFEEZ B B， SIDDIQUI I A， et al. Ligand-Dependent Corepressor Acts as a Novel Androgen Receptor Corepressor， Inhibits Prostate Cancer Growth， and Is Functionally Inactivated by the Src Protein Kinase〔J〕. J Biol Chem，2011，286（43）：37108-37117.

〔16〕 WHITE J H， FERNANDES I， MADER S， et al. Corepressor Recruitment by Agonist-Bound Nuclear Receptors〔J〕. Vitam Horm，2004，68：123-143.

〔17〕 VOGELSANG T L R， SCHMOECKEL E， KUHN C， et al. Regulation of LCoR and RIP140 Expression in Cervical Intraepithelial Neoplasia and Correlation with CIN Progression and Dedifferentiation〔J〕. J Cancer Res Clin Oncol，2020，146（7）：1847-1855.

[1]	Li Jing, Ling Haowei, Shi Kaixuan, Liu Rong, Xiao Chaojiang, Jiang Bei, . Study on Chemical Constituents of the Root of Agapetes lacei var. tomentella [J]. Journal of Dali University, 2023, 8(8): 1-5.
[2]	Ling Haowei, Li Jing, Shi Kaixuan, Liu Rong, Xiao Chaojiang, Jiang Bei, . Study on Chemical Constituents of Gentiana nanobella [J]. Journal of Dali University, 2023, 8(8): 6-10.
[3]	Shi Kaixuan, Li Jing, Ling Haowei, Liu Rong, Xiao Chaojiang, Jiang Bei, . Study on Chemical Constituents of Leptodermis pilosa [J]. Journal of Dali University, 2023, 8(8): 11-14.
[4]	Qin Zikang, Guo Haihui, Shan Fang, Wu Lei, Wang Fusheng. Synthesis of L-5-Hydroxytryptophan Derivatives [J]. Journal of Dali University, 2023, 8(8): 15-20.
[5]	Shan Fang, Guo Haihui, Qin Zikang, Li Chunxu, Wang Fusheng. Synthesis of Gentiournoside D Amide Derivatives [J]. Journal of Dali University, 2023, 8(8): 21-24.
[6]	Lai Yuxia, Guo Haihui, Qin Zikang, Shan Fang, Wang Fusheng. Optimization of Ｓelective Ester Hydrolysis Conditions for Depresteroside [J]. Journal of Dali University, 2023, 8(8): 25-29.
[7]	Lu Xixing, Zhu Ling, Wu Hao, Zhang Chenggui, Yang Yongshou, Chen Zhipeng, Liu Heng, . Chemical Composition Analysis and Multicomponent Content Determination of Extracts of Periplaneta americana from Different Breeding Plants [J]. Journal of Dali University, 2023, 8(8): 30-38.
[8]	Su Baoshun, Liu Zhitong. Preliminary Study on Antibacterial Effect and Active Parts of Dobinea delavayi [J]. Journal of Dali University, 2023, 8(8): 39-43.
[9]	Li Xiaoji, Li Guorong. Construction and Effectiveness of the Pharmaceutical Control System of Injection Proton Pump Inhibitors [J]. Journal of Dali University, 2023, 8(8): 44-48.
[10]	Ye Shengying, Wang Xinrui, Gao Jingwen, Qin Yan. The Protective Effect of Ghrelin on High Glucose-Induced Ｈepatocellular Injury [J]. Journal of Dali University, 2023, 8(8): 49-53.
[11]	Deng Wenbin, Huang Shiqiong, Wang Xue, Li Lihua. Effects of High Salt Intervention on Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cell [J]. Journal of Dali University, 2023, 8(8): 54-58.
[12]	Wu Maofang, Shi Wen, Xiao Shouyong, Cai Jingjing, Liu Zengping, Fang Hong. Propensity Score Matching Analysis of Postoperative Radiotherapy in Patients with Stage ⅢA/N2 Non-Small Cell Lung Carcinoma [J]. Journal of Dali University, 2023, 8(8): 64-70.
[13]	Xiao Min, Xiong Miaomiao, Cheng Mingjing, Cui Xiuyu, Zhao Yuanqi, Zhao Weidong. Scientometric Analysis of Ferroptosis Literature Based on Web of Science #br# [J]. Journal of Dali University, 2023, 8(8): 71-77.
[14]	Rao Yanhua, Wu Xingjun, Quan Yuan, Guan Qingni, Wang Suorong, Tian Ying. Study on the Elderly Care Career-Selection Motivation and Influencing Factors of Vocational Nursing Students [J]. Journal of Dali University, 2023, 8(8): 78-83.
[15]	Ma Yongxin, Ao Bo, Yang Guoqin, He Lixiu, Qian Jinfu. Qualitative Study on Professional Identity and Motivation for Enrollment of Midwifery Undergraduates in Yunnan Province [J]. Journal of Dali University, 2023, 8(8): 84-87.

Expression of RIP140 and LCOR in High-Grade Cervical Intraepithelial Neoplasia

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments