Study on the Mechanism of JAK2-STAT3 Signaling Pathway Mediated by Th1/Th2 Immune Balance Shift in the Occurrence of Infectious Preterm Birth

Abstract

Abstract:

Objective： To investigate the Th1/Th2 immune imbalance and the involvement of the JAK2-STAT3 signaling pathway in the mechanism of infectious preterm birth. Methods： A total of 60 preterm patients treated at the First Affiliated Hospital of Dali University were selected as the research objects（preterm group）. Based on the results of postpartum fetal membrane tissue pathological examination， the preterm group was divided into the infectious preterm birth group and non-infectious preterm birth group. 20 puerperae who had full-term deliveries during the same period were selected as the control group. The expression levels of interferon-γ （IFN-γ）， interleukin-4 （IL-4）， interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in the peripheral blood of the puerperae in the three groups were detected by ELISA kits， and the Th1/Th2 immune regulatory balance index was calculated. The expressions of STAT3 and nuclear factor-κB（NF-κB） proteins in fetal membrane tissue were detected by immunohistochemistry. Results： The expression levels of IFN-γ and TNF-α were significantly elevated in the preterm group（P<0.05）， especially in the infectious preterm birth group， indicating that the Th1/Th2 immune balance had a significant shift toward Th1. The expression levels of IL-6， STAT3， and NF-κB proteins were significantly higher in the infectious preterm birth group（P<0.05）， while there was no significant difference between the non-infectious preterm birth group and the control group（P>0.05）. Conclusion： The Th1/Th2 immune balance may be involved in the occurrence of infectious preterm birth through TNF-α， NF-κB， IL-6 and JAK2-STAT3 signaling pathways.

Key words:

infectious preterm birth, Th1/Th2 immune balance, JAK2-STAT3 signaling pathway

CLC Number:

R714.21 ','1');return false;" target="_blank">
R714.21

Du Lingying, Li Zhonglian, Yang Qiongyan, Si Yanting, Guo Ying.

Study on the Mechanism of JAK2-STAT3 Signaling Pathway Mediated by Th1/Th2 Immune Balance Shift in the Occurrence of Infectious Preterm Birth [J]. Journal of Dali University, 2023, 8(10): 52-58.

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