Isolation and Identification of Bacteriophages Specific to Intestinal Pathogen in the Water of Downstream of Erhai Lake

J4 ›› 2011, Vol. 10 ›› Issue (12): 97-98.

Isolation and Identification of Bacteriophages Specific to Intestinal Pathogen in the Water of Downstream of Erhai Lake

Objective: To investigate the distribution of intestinal bacteria in the downstream of Erhai Lake by isolation and
identification phage of intestinal bacteria. Methods: Plaque method was applied to isolate and identify bacteriophages specific to intestinal pathogens in the water of Erhai Lake. Results: Bacteriophages specific to dysentery bacillus, typhoid bacillus, paratyphoid B bacteria, E. coli were successfully isolated. Conclusion: The downstream water of Erhai Lake was contaminated by dysentery bacillus, typhoid bacillus, paratyphoid B bacteria and E. coli. This suggested that it is necessary to further strengthen the management of surrounding living sewage discharge in Dali City.

School of Basic Medicine, Dali University, Dali, Yunnan 671000, China

Received:2011-10-12 Online:2011-12-15 Published:2011-12-15

Abstract

Abstract:

Key words: intestinal pathogen, Erhai Lake, phage

CLC Number:

R372

LI Jie, YU Ze-Ying, ZHAO Zong-Mei, YAN Yi-Jun, MA Zhi-Gang, LIU Qi. Isolation and Identification of Bacteriophages Specific to Intestinal Pathogen in the Water of Downstream of Erhai Lake[J]. J4, 2011, 10(12): 97-98.

References

〔1〕徐安伦，董保举，刘劲松，等. 洱海湖滨大气边界层结构
及特征分析〔J〕. 高原气象，2010，29（3）：637- 644.
〔2〕程艳，李柄花，此里能布，等. 洱海弥苴河流域降雨侵蚀
力特征研究〔J〕. 中国水土保持，2008（6）：29-33.
〔3〕郭海萍，陈振辉，黄艳梅，等. 污水中肠道杆菌噬菌体的
分离及其生物学活性研究〔J〕. 南方医科大学学报，2007，
27（10）：1545-1546.
〔4〕王梅，海荣. 鼠疫噬菌体研究概述〔J〕. 中国媒介生物学
及控制杂志，2011，22（3）：297-300.
〔5〕苏胜兵，马红霞，徐凤宇. 噬菌体在细菌性疾病诊断和治
疗中的应用〔J〕. 中国兽医科学，2011，41（5）：546-550.
〔6〕Weinbauer M G. Ecology of prokaryotic viruses〔J〕. Fems
Microbiology Reviews，2004，28（2）：127-181.
〔7〕Cairns B J，Timms A R，Jansen V A A，et al. Quantitative
Models of In Vitro Bacteriophage -Host Dynamics and
Their Application to Phage Therapy〔J〕. Plos Pathogens，
2009，5（1）：1-10.
〔8〕刘晓彬，王珂，成伏波，等. 噬菌体对小鼠肠源性铜绿假
单胞菌感染的治疗作用〔J〕. 吉林大学学报：医学版，2009，
35（4）：660-663.
〔9〕温占波，李劲松. 噬菌体治疗细菌性疾病的研究进展〔J〕.
军事医学科学院院刊，2008，32（2）：172-174.
〔10〕成伏波，郑文旭，李菁华，等. 噬菌体疗法对烧伤合并铜
绿假单胞菌感染小鼠的治疗作用〔J〕. 中国感染与化疗
杂志，2009，9（1）：18-21.
〔11〕中华人民共和国传染病防治法〔S〕.
〔12〕徐焰，熊鸿燕，苏明权，等. 医院内污水中噬菌体的及其
生物学特性观察〔J〕. 第四军医大学学报，2003，24（9）：
846-848.
〔13〕焦春香，刘东平，陶景丽，等. 大理洱海水体粪大肠菌群
污染状况初步调查〔J〕.大理学院学报，2010，9（8）：29-
30.

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[2]	Wei Zhuang, Hu Yangyang. Effects of Radical Esophagectomy Combined with Chemoradiotherapy on Clinical Efficacy and Immune#br# Function in Patients with Advanced Esophageal Cancer [J]. Journal of Dali University, 2026, 11(2): 37-41.
[3]	Yang Wenrong, Li Aimin, Yang Lijie, Song Zheng, Li Zhonghua, Yang Wanchun, Cha Jianyuan. Expression Analysis of Acetyltransferase KAT2A and Survivin Protein Acetylation#br# in Esophageal Cancer Tissues [J]. Journal of Dali University, 2025, 10(2): 51-54.
[4]	Hu Xiaokang, , , , , Wang Tao, , , , , Yang Liquan, , , , . On the History of the Scientific Expedition for Cangshan Mountain and Erhai Lake [J]. Journal of Dali University, 2024, 9(6): 1-.
[5]	Chen Youjun , , , . Natural Geography of Cangshan Erhai National Nature Reserve [J]. Journal of Dali University, 2024, 9(6): 19-.
[6]	Song Hong, , Yin Zhilong, , Wang Xun, , Yu Honglei, , Du Zhuangzhuang, , Mao Benyong, , , , Li Miao, , , . Diversity of Tetrigoidea Insects（ Orthoptera: Caelifera） in Cangshan Mountain and Erhai Lake Region [J]. Journal of Dali University, 2024, 9(6): 95-.
[7]	Mao Jinbin, , Wu Sibo, , Xi Mansong, Li Hang, Zhou Jun, , . Diversity and Population Spatiotemporal Pattern of Waterfowl in Erhai Lake [J]. Journal of Dali University, 2024, 9(6): 103-.
[8]	Zhong Chuanyan, , Li Gang , Chu Weile, , Shen Jian, , Feng Jimeng , , Long Xiaowen, , . Diversity of Fish in Erhai Lake [J]. Journal of Dali University, 2024, 9(6): 108-.
[9]	Lei Xiaoyi, Chen Kaijie, He Tian, Deng Zhixin, Lu Xuan, Sui Shiyan. Contents of Nitrogen, Phosphorus, Arsenic and Metals in Soils around Erhai Lake and Their Potential Ecological Risk Assessment#br# [J]. Journal of Dali University, 2024, 9(4): 95-100.
[10]	Zhong Xiaoxia, Zhou Shixiong, Zhang Le, Cai Weiling. Analysis of the Diagnostic Value of Combined Detection of MIP-1β、IL-27 and IL-35 in Tuberculous Pleural Effusion [J]. Journal of Dali University, 2023, 8(10): 72-75.
[11]	Jiang Guomeng, LiaoYing, Zhang Guomei, Liu Qingqing. Application of Transesophageal Three-Dimensional Echocardiography in the Diagnosis of Cardiac Diseases [J]. Journal of Dali University, 2021, 6(10): 60-.
[12]	Yang De'ai. An Anthropological Perspective on the Belief System of Water Gods in Erhai Areas of Dali [J]. Journal of Dali University, 2019, 4(7): 1-7.
[13]	Li Xuelong. A Preliminary Study on the Ancient Practices of the Bai People in Managing#br# the Headwaters of Erhai Lake [J]. Journal of Dali University, 2019, 4(1): 6-9.
[14]	Xu Fang, Liu Taixiang, Shi Xianghuan, Zhang Shuxia*. A Study on the Daytime Wintering Behavior of the Common Coot（Fulica atra）at Erhai Lake [J]. , 2018, 3(6): 90-94.
[15]	Pan Wenliang. A Comparison of Concepts in the Protection of Erhai Lake in Dali and Holy Lakes in Tibet#br# [J]. Journal of Dali University, 2018, 3(1): 43-47.