Abstract:

Objective: To investigate the distribution of genotypes of oral Candida albicans isolated from HIV-related group and
healthy carriers group. Methods: PCR with CA-INT primers was designed to amplify group 1 intron-containing region in 25S rDNA of oral Candida albicans, the different strains of oral Candida albicans were classified into genotypes on the basis of bands from amplicons. Then the genotypic distribution results of all oral Candida albicans were analyzed with SPSS 17.0 by chi-square test. Results: One hundred and eight strains of oral cavities Candida albicans were classified into genotype A, B and C. Genotype A was the most predominant one. Identical genotypes distribution appeared in both groups. But the constituent ratio of genotype A of oral Candida albicans strains in HIV-positive group was statistically higher compared to the healthy people group. Conclusion: Genotypes of oral Candida albicans in both groups were highly polymorphic. The method in text is suitable to apply in genotyping research of oral Candida albicans.

Key words: Candida albicans, HIV, PCR, rDNA genotyping