J4 ›› 2011, Vol. 10 ›› Issue (12): 6-9.
目的：研究美洲大蠊提取物CII-3 对乌头碱致大鼠心律失常的保护作用，并初步探讨其作用机制。方法：采用股静脉注射乌头碱诱发大鼠心律失常，观察CII-3 对乌头碱致VP、VT、VF、CA 用量和VP、VT、VF、CA 发生率及心律失常评分的影响，测定大鼠血清中CK和LDH的活性。结果：一定剂量的C域-3 可以提高乌头碱所致大鼠VP、VT、VF、CA 的用量（P<0.05，0.01），能降低大鼠VT、VF、CA 的发生率及大鼠心律失常评分，降低血清中CK 的活性（P<0.05，0.01），但对LDH 的活性影响甚小（P>0.05）。结论：CII-3 对乌头碱诱导的大鼠心律失常具有一定的保护作用。
Objective: To explore the protective effect and mechanism of CII-3 extracted from Periplaneta americana against
arrhythmia induced by aconitine in rats. Methods: Arrhythmia in rat was induced by aconitine with femoral vein injection, with different doses of CII-3 treatment, the incidence rates of ventricular premature beats（VP）, ventricular tachycardia（VT）, ventricular fibrillation （VF）and cardiac arrest （CA）were recorded, and the arrhythmia score, activity of CK and LDH were detected. Results:The amount of aconitine in inducing VP, VT, VF and CA was increased after CII-3 treatment, which showed decreased incidences of VT, VF, CA and the arrhythmia score, as well as the the activity of CK （P<0.05）. However, there was no influence of CII-3 on the activity of LDH. Conclusion: The data demonstrated that there was remarkable protective effect of CII-3 against arrhythmia induced by aconitine in rats.
摘要：
目的：研究美洲大蠊提取物CII-3 对乌头碱致大鼠心律失常的保护作用，并初步探讨其作用机制。方法：采用股静脉注射乌头碱诱发大鼠心律失常，观察CII-3 对乌头碱致VP、VT、VF、CA 用量和VP、VT、VF、CA 发生率及心律失常评分的影响，测定大鼠血清中CK和LDH的活性。结果：一定剂量的C域-3 可以提高乌头碱所致大鼠VP、VT、VF、CA 的用量（P<0.05，0.01），能降低大鼠VT、VF、CA 的发生率及大鼠心律失常评分，降低血清中CK 的活性（P<0.05，0.01），但对LDH 的活性影响甚小（P>0.05）。结论：CII-3 对乌头碱诱导的大鼠心律失常具有一定的保护作用。
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