J4 ›› 2011, Vol. 10 ›› Issue (12): 97-98.

• 学生园地 • 上一篇    下一篇

洱海下游肠道致病菌噬菌体分离与鉴定

目的:分离洱海下游水体中的肠道致病菌噬菌体,了解肠道致病菌污染情况。方法:通过噬菌斑法从洱海下游水体中分离和鉴定各种肠道致病菌噬菌体。结果:成功分离出了痢疾杆菌噬菌体、伤寒杆菌噬菌体、乙型副伤寒杆菌噬菌体、大肠杆菌噬菌体4 种肠道致病菌噬菌体。结论:洱海下游水体存在痢疾杆菌、伤寒杆菌、乙型副伤寒杆菌、大肠杆菌污染,提示需进一步加强大理周边生活污水排放的治理工作。   

  1. 大理学院基础医学院,云南大理 671000
  • 收稿日期:2011-10-12 出版日期:2011-12-15 发布日期:2011-12-15
  • 作者简介:李洁,2007 级医学检验专业本科学生.
  • 基金资助:

    大理学院大学生科研基金资助项目(KYSX2010-051)

Isolation and Identification of Bacteriophages Specific to Intestinal Pathogen in the Water of Downstream of Erhai Lake

Objective: To investigate the distribution of intestinal bacteria in the downstream of Erhai Lake by isolation and
identification phage of intestinal bacteria. Methods: Plaque method was applied to isolate and identify bacteriophages specific to intestinal pathogens in the water of Erhai Lake. Results: Bacteriophages specific to dysentery bacillus, typhoid bacillus, paratyphoid B bacteria, E. coli were successfully isolated. Conclusion: The downstream water of Erhai Lake was contaminated by dysentery bacillus, typhoid bacillus, paratyphoid B bacteria and E. coli. This suggested that it is necessary to further strengthen the management of surrounding living sewage discharge in Dali City.   

  1. School of Basic Medicine, Dali University, Dali, Yunnan 671000, China
  • Received:2011-10-12 Online:2011-12-15 Published:2011-12-15

摘要:

目的:分离洱海下游水体中的肠道致病菌噬菌体,了解肠道致病菌污染情况。方法:通过噬菌斑法从洱海下游水体中分离和鉴定各种肠道致病菌噬菌体。结果:成功分离出了痢疾杆菌噬菌体、伤寒杆菌噬菌体、乙型副伤寒杆菌噬菌体、大肠杆菌噬菌体4 种肠道致病菌噬菌体。结论:洱海下游水体存在痢疾杆菌、伤寒杆菌、乙型副伤寒杆菌、大肠杆菌污染,提示需进一步加强大理周边生活污水排放的治理工作。

关键词: 肠道致病菌, 洱海, 噬菌体

Abstract:

Objective: To investigate the distribution of intestinal bacteria in the downstream of Erhai Lake by isolation and
identification phage of intestinal bacteria. Methods: Plaque method was applied to isolate and identify bacteriophages specific to intestinal pathogens in the water of Erhai Lake. Results: Bacteriophages specific to dysentery bacillus, typhoid bacillus, paratyphoid B bacteria, E. coli were successfully isolated. Conclusion: The downstream water of Erhai Lake was contaminated by dysentery bacillus, typhoid bacillus, paratyphoid B bacteria and E. coli. This suggested that it is necessary to further strengthen the management of surrounding living sewage discharge in Dali City.

Key words: intestinal pathogen, Erhai Lake, phage

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