异丙肾上腺素致大鼠心肌缺血型慢性心力衰竭的实验研究

J4 ›› 2011, Vol. 10 ›› Issue (2): 18-21.

异丙肾上腺素致大鼠心肌缺血型慢性心力衰竭的实验研究

1.大理学院药学与化学学院，云南大理 671000；2.大理学院基础医学院，云南大理 671000

收稿日期:2011-01-06 出版日期:2011-02-15 发布日期:2011-02-15
作者简介:任海花，硕士研究生，主要从事心血管药理研究.

Experimental Study of Isoproterenol-induced Ischemic Chronic Heart Failure in the Rat

1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China;
2. College of Basic Medicine; Dali University, Dali, Yunnan 671000, China

Received:2011-01-06 Online:2011-02-15 Published:2011-02-15

摘要/Abstract

摘要：

目的：建立异丙肾上腺素（ISO）缺血型慢性心力衰竭（CHF）大鼠模型，为研究慢性心衰的发病机制和有效治疗措施提供理想的动物模型。方法：模型组大鼠首次按3.0 mg/（kg·d）腹腔注射ISO连续3 d，观察4周，第5周按2.0 mg/（kg·d）继续给药2 d，观察1周，第6周再按3.0 mg/（kg·d）给药2 d，1 周后进行血流动力学指标、血清心肌肌钙蛋白-Ⅰ（cTn-Ⅰ）、血管紧张素Ⅱ（AngII）水平等测定和肺组织病理学观察。结果：模型组大鼠较正常对照组左室收缩最大速率降低25.2%，左室舒张最大速率绝对值降低了33.7%，肺组织病理改变明显，血清cTn-Ⅰ和AngII水平明显增加（P＜0.01）。结论：采用大鼠间断性腹腔反复注射一定剂量ISO制备慢性心衰模型方法稳定、可靠、容易控制。

关键词: 慢性心力衰竭, 异丙肾上腺素, 血流动力学, 血管紧张素Ⅱ

Abstract:

Objective: To establish a model of isoproterenol-induced ischemic CHF in the rat, and provide a useful animal model for studies on pathogenesis and effective therapy in CHF. Methods: The model group was injected intraperitoneally（ip）with ISO at 3.0 mg/（kg/d）for 3 consecutive d and then was observed for four weeks. In the fifth week, the model group was injected (ip) sequentially with ISO at 2.0 mg/（kg·d）for 2 consecutive d and then was observed for one week. In the sixth week, the model group was injected （ip）with ISO at 3.0 mg/（kg·d）for2 consecutive d. One week later, the hemodynamic, serum cTn-Ⅰ and Ang II were measured. Lung tissue pathological changes were observed. Results: Model groups rats showed that the value of +dp/dtmax reduced 33.7 percent, and the absolute value of- dp/dtmax reduced 25.2 percent. Lung tissue pathological changes were obvious. The serum cTn- and Ang II increased significantly （P <0.01）. Conclusion: Model of CHF in the rats was induced by ISO multiple and
discontinuity ip of at a certain dose and it is reliable and easy to copy and control.

Key words: chronic heart failure, isoproterenol, hemodynamic, angiotensin II

中图分类号:

R541.6

任海花, 吴建新, 杨玲玲, 苏娟, 钮荣祥. 异丙肾上腺素致大鼠心肌缺血型慢性心力衰竭的实验研究[J]. J4, 2011, 10(2): 18-21.

REN Hai-Hua, WU Jian-Xin, YANG Ling-Ling, SU Juan, NIU Rong-Xiang. Experimental Study of Isoproterenol-induced Ischemic Chronic Heart Failure in the Rat[J]. J4, 2011, 10(2): 18-21.

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