J4 ›› 2012, Vol. 11 ›› Issue (9): 25-28.
目的：提高扇贝贝壳有效利用的附加值。方法：使用分化成熟的3T3-L1脂肪细胞，添加扇贝贝壳的甲醇抽出成分，测定
3T3-L1脂肪细胞内脂肪分解释放出的甘油含量；并对3T3-L1脂肪细胞进行Oil Red“O”染色，直观脂肪细胞内脂肪的染色情况，评价扇贝贝壳所含生物活性物质对脂肪分解的促进作用。结果：添加了扇贝贝壳的甲醇抽出成分对3T3-L1脂肪细胞释放出的甘油与对照组相比显示出增加的现象，并具有统计学意义（P<0.05）；细胞染色结果直观显示出细胞内脂肪滴的明显减少。结论：扇贝贝壳所含生物活性物质有促进脂肪分解的作用，提示了扇贝贝壳有效利用的新途径。
Objective: To investigate the effect of scallop shells on fat degradation and find new ways to increase their added value.
Methods: To evaluate the effect of bioactive substances of scallop shells on fat degradation, 3T3-L1 cells were treated with methanol extract of scallop shells, the amounts of glycerol secreted from 3T3-L1 cells were measured and the fat contents in 3T3-L1 cells were observed by Oil Red O staining. Results: 3T3-L1 cells treated with scallop shell extract secreted more glycerol (P<0.05) and were with less fat. Conclusion: This study provided evidence that bioactive substances in scallop shells promote fat degradation, which suggests a potential value of scallop shells.
摘要：
目的：提高扇贝贝壳有效利用的附加值。方法：使用分化成熟的3T3-L1脂肪细胞，添加扇贝贝壳的甲醇抽出成分，测定
3T3-L1脂肪细胞内脂肪分解释放出的甘油含量；并对3T3-L1脂肪细胞进行Oil Red“O”染色，直观脂肪细胞内脂肪的染色情况，评价扇贝贝壳所含生物活性物质对脂肪分解的促进作用。结果：添加了扇贝贝壳的甲醇抽出成分对3T3-L1脂肪细胞释放出的甘油与对照组相比显示出增加的现象，并具有统计学意义（P<0.05）；细胞染色结果直观显示出细胞内脂肪滴的明显减少。结论：扇贝贝壳所含生物活性物质有促进脂肪分解的作用，提示了扇贝贝壳有效利用的新途径。
中图分类号:
