大理地区健康人阴道念珠菌体外药物敏感性研究

J4 ›› 2012, Vol. 11 ›› Issue (9): 41-43.

大理地区健康人阴道念珠菌体外药物敏感性研究

1.大理学院基础医学院，云南大理 671000；2.大理学院公共卫生学院，云南大理 671000

收稿日期:2012-05-11 修回日期:2012-05-28 出版日期:2012-09-15 发布日期:2012-09-15
作者简介:何伟，临床医学专业2007级本科生.

In vitro Study on Antifungal Susceptibility of Vaginal Candida from Healthy Population in Dali

1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China;
2. College of Public Health, Dali University, Dali, Yunnan 671000, China

Received:2012-05-11 Revised:2012-05-28 Online:2012-09-15 Published:2012-09-15

摘要/Abstract

摘要：

目的：通过对分离自大理地区健康女性阴道念珠菌的药物敏感性分析，为临床抗真菌药物的选择提供依据。方法：采用
微量液基稀释法，测定念珠菌对氟康唑（FCZ）、酮康唑（KCZ）、5-氟胞嘧啶（5-FC）和两性霉素B（AMB）4种常用抗真菌药物的敏感性（MIC值）。结果：45株健康人群阴道念珠菌对抗真菌药物FCZ、KCZ、5-FC、AMB的耐药率分别为：8.89%、6.67%、6.67%、2.22%，敏感率分别为：80%、84.44%、91.11%、97.78%，1株克柔念珠菌对FCZ、KCZ和5-FC同时耐药，另1株克柔念珠菌对KCZ和5-FC同时耐药。45株念珠菌对四种抗真菌药物的耐药率差异无统计学意义，敏感率除AMB的药物敏感性比FCZ好（P=0.008），AMB的药物敏感性比KCZ的好（P=0.027），其余药物的敏感率差异无统计学意义（P跃0.05）。结论：上述4种抗真菌药物均可选作阴道念珠菌病的治疗，相比而言，更推荐使用AMB，但应注意某些菌株的剂量依赖性敏感、耐药及交叉耐药现象。

关键词: 药物敏感性, 念珠菌, 健康人群

Abstract:

Objective: To provide a basis for clinical selection of antifungal drugs through vaginal candida antifungal susceptibility
test. Methods: We measured the vaginal candida antifungal susceptibility (minimal inhibitory concentration, MIC) to fluconazole (FCZ), ketoconazole (KCZ), 5-fluorocytosine (5-FC) and amphotericin B (AMB) by using Broth microdilution method. Results: The resistance rates of 45 strains vaginal candida from healthy subjects to antifungal drug FCZ, KCZ, 5-FC, AMB were 8.89%, 6.67%,6.67% and 2.22% respectively. And the sensitive rates were 80%,84.44%, 91.11%, 97.78% respectively. One strain of C. krusei is resistant to FCZ, KCZ and 5-FC while another strain was simultaneous resistant to KCZ and 5-FC. There were no significance differences in all 45 strains of candida in antifungal drugs resistant rate. The sensitivity of Candida to AMB was higher than that of FCZ (P=0.008). The sensitivity of Candida to AMB was higher than that of KCZ (P=0.027). There were no significant differences among rest drugs in sensitive rate (P>0.05). Conclusion: The above four kinds of commonly used clinical antifungal drugs can be selected to treat vaginal candidasis, but should be aware that some strains are sensitive and resistant with dose-dependent manner.

Key words: antifungal susceptibility, candida, healthy subject

中图分类号:

R379.4

何伟, 陈财发, 姚毅, 朱文, 杨泽丽, 白丽. 大理地区健康人阴道念珠菌体外药物敏感性研究[J]. J4, 2012, 11(9): 41-43.

HE Wei, CHEN Cai-Fa, YAO Yi, ZHU Wen, YANG Ze-Li, BAI Li. In vitro Study on Antifungal Susceptibility of Vaginal Candida from Healthy Population in Dali[J]. J4, 2012, 11(9): 41-43.

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