NF-κB在肝癌介入治疗后癌旁肝细胞中的表达

J4 ›› 2014, Vol. 13 ›› Issue (10): 42-46.

NF-κB在肝癌介入治疗后癌旁肝细胞中的表达

大理学院附属医院，云南大理 671000

收稿日期:2014-01-10 出版日期:2014-10-15 发布日期:2014-10-15
作者简介:张靓，硕士研究生，主要从事介入放射学研究.
基金资助:
国家自然科学基金资助项目（81241131）；云南
省教育厅科学研究基金资助项目（2013J113）

NF-кB Expression in Liver Carcinoma Tissues and Tissues Adjacent to Liver Cancer Tissues Post TACE

Affiliated Hospital of Dali University, Dali, Yunnan 671000, China

Received:2014-01-10 Online:2014-10-15 Published:2014-10-15

摘要/Abstract

摘要：

目的：探讨NF-кB在肝癌介入治疗后肿瘤组织和癌旁肝细胞中的表达及其变化特点。方法：建立VX2肝癌模型兔59
只，分为A组（肝癌组）29只，B组（肝癌经导管肝动脉栓塞介入组）30只、C组（对照组）20只，免疫组化检测各组肝癌及癌旁肝
组织中的NF-кB表达。结果：A组肝癌组织与癌旁肝组织NF-κB阳性表达率分别为72.4%（21/29）、37.9%（11/29），肝癌介入治
疗后肝癌组织和癌旁组织NF-κB阳性表达率82.4%（24/30）、60%（18/30），C组NF-κB阳性表达率为10%（2/20），各组与对照比
较，除A组癌旁肝组织外差异均有统计学意义（P < 0.05），其余两两比较差异无统计学意义（P > 0.05）。结论：治疗前癌旁组
织，其内NF-кB的表达水平尚处于较低水平，介入治疗后的癌旁肝组织的脂肪变性及炎性反应等因素可能会导致NF-κB的表
达增加。

关键词: NF-кB, 肝癌, 介入治疗

Abstract:

Objective: To explore the expression of NF-кB in Liver carcinoma tissues and adjacent noncancerous liver tissue post
interventional therapy and its characteristic changes. Methods: 59 rabbits with VX2 carcinoma model were randomly divided into 2
groups, 29 in group A (liver cancer), and 30 in group B (liver cancer/transcatheter hepatic arterial embolization, TACE) as a study
group. And in group C 20 rabbits with no liver cancer as a control group. Immunohistochemical detection of NF-кB in liver cancer
tissue and normal tissues adjacent to liver cancer tissue was done. Results: In group A, positive expression rate of NF-κB was 72.4%
(21/29) in cancerous liver tissue and 37.9 %( 11/29) in liver tissue adjacent to carcinoma. In group B (post interventional therapy)
positive expression rate of NF-κB was 82.4% (24/30) in liver cancer tissues and 60% (18/30) in adjacent tissues. In group C, NF-κB
positive expression rate was 10% (2/20). Then each group was compared with control. Except in liver tissue adjacent to carcinoma in
group A, the data recorded was statistically significant (P < 0.05). The other two data had no statistical significance (P > 0.05).
Conclusion: The expression level of NF-κB is relatively low before interventional therapy in the tissue adjacent to carcinoma. Post
interventional therapy. There is increased expression of NF- κB, may be due to the fatty degeneration of liver tissues adjacent to
carcinoma and inflammatory reaction.

Key words: NF-кB, liver carcinoma, interventional therapy

中图分类号:

R735.7

张靓，杜伟. NF-κB在肝癌介入治疗后癌旁肝细胞中的表达[J]. J4, 2014, 13(10): 42-46.

ZHANG Liang, DU Wei. NF-кB Expression in Liver Carcinoma Tissues and Tissues Adjacent to Liver Cancer Tissues Post TACE[J]. J4, 2014, 13(10): 42-46.

参考文献

〔1〕杜伟，袁贵斌，汪永平，等. 介入法植入国产支架治疗前
列腺增生症的临床应用〔J〕. 大理学院学报，2010，9（2）:
47-49.
〔2〕王守峰，张金岭，殷忠美，等. 不同Child 分级的高HB?
VDNA 滴度原发性肝癌介入治疗后肝功能损害的对比
研究〔J〕. 中国肿瘤外科杂志，2013，5（3）：160-162.
〔3〕独建库，李冠海，张明德，等. 肝癌1063 例介入治疗并
发症处理的研究〔J〕. 临床军医杂志，2011，39（3）:491-
493.
〔4〕SEN R，BALTIMORE D. Multiple nuclear factors interact
with the immunoglobulin enhancer Sequences〔J〕. Cell，
1986，46（5）:705-716.
〔5〕AMEYA PARANJPE，KALKUNTE S，SRIVENUGOPAL.
Degradation of NF- κB，p53 and other regulatory redoxsensitive
proteins by thiolconjugating and nitrosylating
drugs in human tumor cells〔J〕. Carcinogenesis，2013，34
（5）:990-1000.
〔6〕范伟，刘朝晖，张溪林，等. 慢性阻塞性肺疾病大鼠骨骼
肌中NF-кB 通路的研究〔J〕. 中国医学创新，2012，9
（18）:15-17.
〔7〕AMIRI K I，RICHMOND A. Role of nuclear factor-kappa
B in melanoma〔J〕. Cancer Metastasis Rev，2005，24: 301-
313．
〔8〕KALALTZIDIS D，GILMORE T D. Transcription factor
cross- talk: the estrogen receptor and NF- kappa B〔J〕.
Trends Endocrinol Metab，2005，16:46-52.
〔9〕HUAN DENG，ZHENZHEN ZHOU，WEI TU，et al. AEG1
induces COX- 2 expression via activation of NF- κB and
AP- 1 in hepatoma HepG2 cells〔J〕. Chinese- German J
Clin Oncol，2013，12（6）:253-256.
〔10〕KUO M T，LIU Z，WEI Y，et al. Induction of human
MDRl gene expression by 2-acelylaminofluoxene is medi?
ated by effectors of the phosphoinositide 3-kinase Path?
way that activate NF- kappa signaling〔J〕. Oncogene，
2002，21（13）:1945-1954.
〔11〕齐京鹏，廖和和，王永峰，等. 不能手术的原发性肝癌
介入治疗与介入加放疗的疗效比较〔J〕. 现代肿瘤医
学，2011，19（3）:528-530.
〔12〕陈爱云. 原发性肝癌介入手术治疗40 例围术期护
理〔J〕. 齐鲁护理杂志，2012，18（20）:66-67.
〔13〕康建荣. 75 例肝癌介入术后并发症的观察及护理〔J〕.
中国实用医药，2012，7（7）:216-217.
〔14〕杨玉琴. 膀胱癌术后化疗灌注的体会〔J〕. 大理学院学
报，2012，11（12）:72-73.
〔15〕LAVON I，PIKARSKY E，GUTKOVICH E，et a1. Nucle?
ar fator-kappaB Protects the liver against genotoxic stress
and functions independently of p53〔J〕. Cancer Res，
2003，63（1）: 25-30.
〔16〕AE-RI N，KATINKA P Z，PAUL P T，et al. Non-canoni?
cal NF-кB signaling in endothelial cells may enhance sy?
novial inflammation by stimulating angiogenesis〔J〕. Jour?
nal of Translational Medicine，2011，9（2）:50.

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